ArticleThe Journal of physiology1998
Functional interactions between nicotinic and P2X channels in short-term cultures of guinea-pig submucosal neurons.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 95 citations in OpenAlex.
- Purinergic signaling in the gut neuro-glial-immune axis: mechanisms of homeostasis, inflammation, and gastrointestinal dysfunction.Frontiers in gastroenterology (Lausanne, Switzerland) · 2026Review
- Anti-inflammatory Therapy by Cholinergic and Purinergic Modulation in Multiple Sclerosis Associated with SARS-CoV-2 Infection.Molecular neurobiology · 2021Review
- Function of P2X4 Receptors Is Directly Modulated by a 1:1 Stoichiometric Interaction With 5-HTFrontiers in cellular neuroscience · 2020Article
- Physiological Concentrations of Zinc Have Dual Effects on P2X Myenteric Receptors of Guinea Pig.Cellular and molecular neurobiology · 2018Article
- Anti-Hu antibodies activate enteric and sensory neurons.Scientific reports · 2016Article
- P2X receptors.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- A New Mechanism of Receptor Targeting by Interaction between Two Classes of Ligand-Gated Ion Channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Purinergic signalling in the gastrointestinal tract and related organs in health and disease.Purinergic signalling · 2014Review
- Review
- Molecular and functional properties of P2X receptors--recent progress and persisting challenges.Purinergic signalling · 2012Review
- A possible role of the cholinergic and purinergic receptor interaction in the regulation of the rat urinary bladder function.Journal of muscle research and cell motility · 2012Article
- Neuronal P2X2 receptors are mobile ATP sensors that explore the plasma membrane when activated.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Interplay between ionotropic receptors modulates inhibitory synaptic strength.Communicative & integrative biology · 2011Article
- Plasticity of non-adrenergic non-cholinergic bladder contractions in rats after chronic spinal cord injury.Brain research bulletin · 2011Article
- Interaction of alpha1D-adrenergic and P2X(7) receptors in the rat lacrimal gland and the effect on intracellular [Ca2+] and protein secretion.Investigative ophthalmology & visual science · 2011Article
- Cross-talk between P2X4 and gamma-aminobutyric acid, type A receptors determines synaptic efficacy at a central synapse.The Journal of biological chemistry · 2011Article
- Molecular mechanisms of cross-inhibition between nicotinic acetylcholine receptors and P2X receptors in myenteric neurons and HEK-293 cells.Neurogastroenterology and motility · 2010Article
- Activation of cholinergic receptors blocks non-adrenergic non-cholinergic contractions in the rat urinary bladder.Brain research bulletin · 2008Article
- Purinergic receptors and synaptic transmission in enteric neurons.Purinergic signalling · 2008Article
- Interaction of P2 purinergic receptors with cellular macromolecules.Naunyn-Schmiedeberg's archives of pharmacology · 2008Review
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3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Functional interactions between nicotinic and P2X receptors in submucosal neurons were investigated. Whole-cell currents induced by ACh (IACh) and ATP (IATP) were blocked by hexamethonium and PPADS), respectively. Currents induced by simultaneous application of the two transmitters (IACh+ATP) were only as large as the current induced by the most effective of these substances. This current occlusion indicates that activation of nicotinic and P2X channels is not independent. 2. Kinetic parameters of IACh+ATP indicate that they are carried through channels activated by either substance. In agreement with this interpretation, both IACh and IATP amplitudes were decreased when ATP and ACh were applied simultaneously, whereas no cross-desensitization was observed when nicotinic and P2X receptors were desensitized individually. 3. Current occlusion was observed at membrane potentials of -60 and +10 mV, when IACh and IATP were inward. However, when these currents were outward (at +40 mV), current occlusion was not observed. Current occlusion was still observed at +40 mV in experiments in which the reversal potential of these currents had been adjusted to more positive values. 4. Current occlusion occurred as soon as currents were detected (< 5 ms), was still present in the absence of Ca2+, Na+ or Mg2+, and after adding staurosporine, genistein, K-252a, or N-ethylmaleimide to the pipette solution. Similar observations were noted after substituting alpha, beta-methylene ATP for ATP, or GTP for GTP-gamma-S in the pipette and in experiments carried out at 36, 23 and 9 C. 5. We propose that nicotinic and P2X channels are in functional clusters of at least two, and that the influx of ions through one activates (through allosteric interactions) a mechanism that inhibits the other channel.
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