ArticleJournal of assisted reproduction and genetics1998
Improving the fixation method for preimplantation genetic diagnosis by fluorescent in situ hybridization.
Article in Journal of assisted reproduction and genetics, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Application of improved single blastomere fixation technique in preimplantation genetic diagnosis.Cytotechnology · 2020Article
- Outcomes of Preimplantation Genetic Diagnosis Cycles by FluorescentChinese medical journal · 2018Article
- A pre-breeding screening program for transgenic boars based on fluorescence in situ hybridization assay.Transgenic research · 2014Article
- Somatic mosaicism in Fanconi anemia: evidence of genotypic reversion in lymphohematopoietic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
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Authors and funding
5 authors.
Funding
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Abstract
purposeOur purpose was to modify a fixation method using Tween-20 and HCl (TH) and to compare it with a protocol using methanol and acetic acid (MA) for the improvement of preimplantation genetic diagnosis by fluorescence in situ hybridization (FISH).
methodsSingle blastomeres were allocated to either the TH or the MA procedure. The two methods were compared to evaluate efficiency of fixation and the intensities of FISH signals.
resultsWith the TH method, 123 (93.9%) of 131 blastomeres were fixed, while only 95 (78.5%) of 121 were fixed with MA. Average scores for the intensity of FISH signals were significantly stronger for TH than for MA (P < 0.05). There was also a significant difference in signal intensity scores between the two methods for type-3 nuclei.
conclusionsOur results indicate that not only are fewer nuclei lost during fixation but also stronger FISH signals can be obtained with the TH method. Thus, modified TH can improve the overall efficiency of preimplantation genetic diagnosis.
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