ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1998
Survival of purified rat photoreceptors in vitro is stimulated directly by fibroblast growth factor-2.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 127 citations in OpenAlex.
- Analysis from the perspective of cilia: the protective effect of PARP inhibitors on visual function during light-induced damage.International ophthalmology · 2020Pooled it
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- Rodent Models of Retinal Degeneration: From Purified Cells in Culture to Living Animals.Cold Spring Harbor perspectives in medicine · 2024Review
- Sequential Fabrication of a Three-Layer Retina-like Structure.Gels (Basel, Switzerland) · 2024Article
- Transplanted human photoreceptors transfer cytoplasmic material but not to the recipient mouse retina.Stem cell research & therapy · 2024Article
- Evaluation of mesenchymal stem cells as anFrontiers in cell and developmental biology · 2024Review
- Pigment epithelium-derived factor (PEDF) and derived peptides promote survival and differentiation of photoreceptors and induce neurite-outgrowth in amacrine neurons.Journal of neurochemistry · 2021Article
- Cerebellar Morphology and Behavioral Profiles in Mice Lacking Heparan SulfateJournal of developmental biology · 2020Article
- Biotechnology and Biomaterial-Based Therapeutic Strategies for Age-Related Macular Degeneration. Part II: Cell and Tissue Engineering Therapies.Frontiers in bioengineering and biotechnology · 2020Review
- Organ Cultures for Retinal Diseases.Frontiers in neuroscience · 2020Review
- Limitations and Promise of Retinal Tissue From Human Pluripotent Stem Cells for Developing Therapies of Blindness.Frontiers in cellular neuroscience · 2020Review
- Article
- Strong upregulation of inflammatory genes accompanies photoreceptor demise in canine models of retinal degeneration.PloS one · 2017Article
- The (Na(+)/K (+))-ATPase activity in the developing rat retina: the role of insulin-like growth factor-I (IGF-I).Cellular and molecular neurobiology · 2015Article
- Vibratome sectioning mouse retina to prepare photoreceptor cultures.Journal of visualized experiments : JoVE · 2014Article
- The ETS transcription factor Etv1 mediates FGF signaling to initiate proneural gene expression during Xenopus laevis retinal development.Mechanisms of development · 2014Article
- The hormone prolactin is a novel, endogenous trophic factor able to regulate reactive glia and to limit retinal degeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Basic fibroblast growth factor expression is implicated in mesenchymal stem cells response to light-induced retinal injury.Cellular and molecular neurobiology · 2013Article
- Muller glia in retinal innate immunity: a perspective on their roles in endophthalmitis.Critical reviews in immunology · 2013Review
- Changes in fibroblast growth factor-2 and FGF receptors in the frog visual system during optic nerve regeneration.Journal of chemical neuroanatomy · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Basic fibroblast growth factor (FGF-2) influences the differentiation and survival of retinal photoreceptors in vivo and in vitro, but it is not known whether it acts directly on photoreceptor FGF receptors or indirectly through activation of surrounding cells. To clarify the effects of FGF-2 on photoreceptor survival, we developed a purified photoreceptor culture system. The outer nuclear layers of postnatal day 5-15 rat retinas were isolated by vibratome sectioning, and the photoreceptor fractions obtained were enzymatically dissociated. Photoreceptors were maintained in monolayer culture for 1 week in a chemically defined medium. Immunocytochemical labeling showed that >99.5% of cells were photoreceptors, and glial contamination represented approximately 0. 2%. Photoreceptors from postnatal day 5-9 retinas survived for at least 24 hr in vitro, whereas cells from postnatal day 10-15 retinas died rapidly. Subsequent studies performed with postnatal day 5 photoreceptors showed that their survival was increased in a dose-dependent manner after the addition of FGF-2. In control cultures, 36% of originally seeded photoreceptors were alive after 5 d in vitro, and in the presence of 20 ng/ml FGF-2 this number was doubled to 62%. This increase was not caused by proliferation of photoreceptor precursors. Denaturing or blocking FGF-2 prevented enhancement of survival. Conversely, only 25.5% of photoreceptors survived in the presence of epidermal growth factor (EGF). FGF- and EGF-receptor mRNA and proteins were detected in purified photoreceptors in vitro, and addition of FGF-2 or EGF led to tyrosine phosphorylation of photoreceptor proteins. These data support a direct mechanism of action for FGF-2 stimulation of photoreceptor survival.
Indexed as
Identifiers
9822727PMC6793308W1545939145What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.