ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1998
Single-cell RT-PCR and functional characterization of Ca2+ channels in motoneurons of the rat facial nucleus.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 57 citations in OpenAlex.
- Article
- Functional Postnatal Maturation of the Medial Olivocochlear Efferent-Outer Hair Cell Synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Developmental Synaptic Changes at the Transient Olivocochlear-Inner Hair Cell Synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Sympathomimetics regulate neuromuscular junction transmission through TRPV1, P/Q- and N-type CaMolecular and cellular neurosciences · 2019Article
- Voltage-gated calcium channels are abnormal in cultured spinal motoneurons in the G93A-SOD1 transgenic mouse model of ALS.Neurobiology of disease · 2016Article
- In vivo impact of presynaptic calcium channel dysfunction on motor axons in episodic ataxia type 2.Brain : a journal of neurology · 2016Article
- Short-term plasticity and modulation of synaptic transmission at mammalian inhibitory cholinergic olivocochlear synapses.Frontiers in systems neuroscience · 2014Review
- Multiple receptor tyrosine kinases are expressed in adult rat retinal ganglion cells as revealed by single-cell degenerate primer polymerase chain reaction.Upsala journal of medical sciences · 2010Article
- Cloning and characterization of voltage-gated calcium channel alpha1 subunits in Xenopus laevis during development.Developmental dynamics : an official publication of the American Association of Anatomists · 2009Article
- Distinct roles of Galpha(q) and Galpha11 for Purkinje cell signaling and motor behavior.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Direct and remote modulation of L-channels in chromaffin cells: distinct actions on alpha1C and alpha1D subunits?Molecular neurobiology · 2004Review
- Voltage-gated Na+ channel activation induces both action potentials in utricular hair cells and brain-derived neurotrophic factor release in the rat utricle during a restricted period of development.The Journal of physiology · 2003Article
- Preservation of motor neuron Ca2+ channel sensitivity to insulin-like growth factor-1 in brain motor cortex from senescent rat.The Journal of physiology · 2003Article
- A-, T-, and H-type currents shape intrinsic firing of developing rat abducens motoneurons.The Journal of physiology · 2003Article
- Synaptic control of motoneuronal excitability.Physiological reviews · 2000Review
- Developmental changes in calcium channel types mediating central synaptic transmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Calcium dynamics and buffering in motoneurones of the mouse spinal cord.The Journal of physiology · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Voltage-dependent Ca2+ channels are a major pathway for Ca2+ entry in neurons. We have studied the electrophysiological, pharmacological, and molecular properties of voltage-gated Ca2+ channels in motoneurons of the rat facial nucleus in slices of the brainstem. Most facial motoneurons express both low voltage-activated (LVA) and high voltage-activated (HVA) Ca2+ channel currents. The HVA current is composed of a number of pharmacologically separable components, including 30% of N-type and approximately 5% of L-type. Despite the dominating role of P-type Ca2+ channels in transmitter release at facial motoneuron terminals described in previous studies, these channels were not present in the cell body. Remarkably, most of the HVA current was carried through a new type of Ca2+ channel that is resistant to toxin and dihydropyridine block but distinct from the R-type currents described in other neurons. Using reverse transcription followed by PCR amplification (RT-PCR) with a powerful set of primers designed to amplify all HVA subtypes of the alpha1-subunit, we identified a highly heterogeneous expression pattern of Ca2+ channel alpha1-subunit mRNA in individual neurons consistent with the Ca2+ current components found in the cell bodies and axon terminals. We detected mRNA for alpha1A in 86% of neurons, alpha1B in 59%, alpha1C in 18%, alpha1D in 18%, and alpha1E in 59%. Either alpha1A or alpha1B mRNAs (or both) were present in all neurons, together with various other alpha1-subunit mRNAs. The most frequently occurring combination was alpha1A with alpha1B and alpha1E. Taken together, these results demonstrate that the Ca2+ channel pattern found in facial motoneurons is highly distinct from that found in other brainstem motoneurons.
Indexed as
Identifiers
9822718PMC6793322W1920274077What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.