ArticleThe EMBO journal1998
C- to N-terminal translocation of preproteins into mitochondria.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- Article
- Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.Nature methods · 2013Article
- Role of polynucleotide kinase/phosphatase in mitochondrial DNA repair.Nucleic acids research · 2012Article
- Twinkle, the mitochondrial replicative DNA helicase, is widespread in the eukaryotic radiation and may also be the mitochondrial DNA primase in most eukaryotes.Journal of molecular evolution · 2006Article
- Identification of sequences required for the import of human protoporphyrinogen oxidase to mitochondria.The Biochemical journal · 2004Article
- Article
- The three modules of ADP/ATP carrier cooperate in receptor recruitment and translocation into mitochondria.The EMBO journal · 2001Article
- Connection of the mitochondrial outer and inner membranes by Fzo1 is critical for organellar fusion.The Journal of cell biology · 2001Article
- Protein import into plant mitochondria: precursor proteins differ in ATP and membrane potential requirements.Plant molecular biology · 2001Article
- An alternate form of Ku80 is required for DNA end-binding activity in mammalian mitochondria.Nucleic acids research · 2000Article
- Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria.The Journal of cell biology · 2000Article
- Supercomplexes in the respiratory chains of yeast and mammalian mitochondria.The EMBO journal · 2000Article
- Isolation and characterization of a Paracentrotus lividus cDNA encoding a stress-inducible chaperonin.Cell stress & chaperones · 2000Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear-encoded mitochondrial matrix proteins in most cases contain N-terminal targeting signals and are imported in a linear N- to C-terminal (N-->C) fashion. We asked whether import can also occur in a C- to N-terminal direction (C-->N). We placed targeting signals at the C-terminus of passenger proteins. Import did occur in this 'backwards' fashion. It paralleled that of the 'normal' N-->C mechanism in terms of efficiency, rate, energetic requirements and ability to mediate unfolding and refolding during and following import of protein containing a folded domain. Furthermore, this reaction was mediated by the TIM17-23 machinery. The import pathway taken by certain inner-membrane proteins contains elements of such a C-->N translocation pathway, as they are targeted to mitochondria by internal targeting signals. These internal targeting signals appear to form loop structures together with neighbouring transmembrane segments, and penetrate the inner membrane in a membrane-potential-dependent manner. The dimeric TIM17-23 complex, together with mt-Hsp70, acts on both sides of the loop structure to facilitate their translocation into the matrix. On one side of the loop import occurs in the common N-->C direction, whereas the translocation of the other side involves the novel C-->N import direction. We conclude therefore that the mitochondrial import machinery displays no preference for the directionality of the import process.
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