ArticleMolecular and cellular biology1998
The evolutionarily conserved eukaryotic arginine attenuator peptide regulates the movement of ribosomes that have translated it.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 61 citations in OpenAlex.
- Structure-based virtual screening of unbiased and RNA-focused libraries to identify new ligands for the HCV IRES model system.RSC medicinal chemistry · 2024Article
- Alternative Transcription Start Site Usage and Functional Implications in Pathogenic Fungi.Journal of fungi (Basel, Switzerland) · 2022Review
- E-site drug specificity of the human pathogenScience advances · 2022Article
- Smart-ORF: a single-molecule method for accessing ribosome dynamics in both upstream and main open reading frames.Nucleic acids research · 2021Article
- Conserved Upstream Open Reading Frame Nascent Peptides That Control Translation.Annual review of genetics · 2020Review
- Ribosome pausing, a dangerous necessity for co-translational events.Nucleic acids research · 2020Review
- Polyamine Control of Translation Elongation Regulates Start Site Selection on Antizyme Inhibitor mRNA via Ribosome Queuing.Molecular cell · 2018Article
- The cell free protein synthesis system from the model filamentous fungus Neurospora crassa.Methods (San Diego, Calif.) · 2018Article
- A gapless genome sequence of the fungus Botrytis cinerea.Molecular plant pathology · 2017Article
- Circadian clock regulation of mRNA translation through eukaryotic elongation factor eEF-2.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Review
- Codon Usage Influences the Local Rate of Translation Elongation to Regulate Co-translational Protein Folding.Molecular cell · 2015Article
- The stringency of start codon selection in the filamentous fungus Neurospora crassa.The Journal of biological chemistry · 2013Article
- The arginine attenuator peptide interferes with the ribosome peptidyl transferase center.Molecular and cellular biology · 2012Article
- Arginine changes the conformation of the arginine attenuator peptide relative to the ribosome tunnel.Journal of molecular biology · 2012Article
- DAZAP1, an RNA-binding protein required for development and spermatogenesis, can regulate mRNA translation.RNA (New York, N.Y.) · 2011Article
- Sequence requirements for ribosome stalling by the arginine attenuator peptide.The Journal of biological chemistry · 2010Article
- Alterations of the 5'untranslated region of SLC16A12 lead to age-related cataract.Investigative ophthalmology & visual science · 2010Article
- The h subunit of eIF3 promotes reinitiation competence during translation of mRNAs harboring upstream open reading frames.RNA (New York, N.Y.) · 2010Article
- her-2 upstream open reading frame effects on the use of downstream initiation codons.Biochemical and biophysical research communications · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Translation of the upstream open reading frame (uORF) in the 5' leader segment of the Neurospora crassa arg-2 mRNA causes reduced initiation at a downstream start codon when arginine is plentiful. Previous examination of this translational attenuation mechanism using a primer-extension inhibition (toeprint) assay in a homologous N. crassa cell-free translation system showed that arginine causes ribosomes to stall at the uORF termination codon. This stalling apparently regulates translation by preventing trailing scanning ribosomes from reaching the downstream start codon. Here we provide evidence that neither the distance between the uORF stop codon and the downstream initiation codon nor the nature of the stop codon used to terminate translation of the uORF-encoded arginine attenuator peptide (AAP) is important for regulation. Furthermore, translation of the AAP coding region regulates synthesis of the firefly luciferase polypeptide when it is fused directly at the N terminus of that polypeptide. In this case, the elongating ribosome stalls in response to Arg soon after it translates the AAP coding region. Regulation by this eukaryotic leader peptide thus appears to be exerted through a novel mechanism of cis-acting translational control.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.