ArticleMolecular and cellular biology1998
Protein kinase A-dependent derepression of the human prodynorphin gene via differential binding to an intragenic silencer element.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 101 citations in OpenAlex.
- Emerging Role of DREAM in Healthy Brain and Neurological Diseases.International journal of molecular sciences · 2023Review
- Mass Spectrometry-Based Structural Proteomics for Metal Ion/Protein Binding Studies.Biomolecules · 2022Review
- Global Gene Knockout ofFrontiers in molecular neuroscience · 2019Article
- Neuronal Expression of Opioid Gene is Controlled by Dual Epigenetic and Transcriptional Mechanism in Human Brain.Cerebral cortex (New York, N.Y. : 1991) · 2018Article
- REM sleep deprivation induces changes of down regulatory antagonist modulator (DREAM) expression in the ventrobasal thalamic nuclei of sprague-dawley rats.Journal of physiology and biochemistry · 2014Article
- The transcription factor DREAM represses the deubiquitinase A20 and mediates inflammation.Nature immunology · 2014Article
- Molecular structure and target recognition of neuronal calcium sensor proteins.Biochimica et biophysica acta · 2012Review
- The dynorphin/κ-opioid receptor system and its role in psychiatric disorders.Cellular and molecular life sciences : CMLS · 2012Review
- Molecular structure and target recognition of neuronal calcium sensor proteins.Frontiers in molecular neuroscience · 2012Article
- Reduced Mid1 Expression and Delayed Neuromotor Development in daDREAM Transgenic Mice.Frontiers in molecular neuroscience · 2012Article
- Cell-specific effects of variants of the 68-base pair tandem repeat on prodynorphin gene promoter activity.Addiction biology · 2011Article
- Tissue-specific DNA methylation of the human prodynorphin gene in post-mortem brain tissues and PBMCs.Pharmacogenetics and genomics · 2011Article
- The DREAM protein is associated with thyroid enlargement and nodular development.Molecular endocrinology (Baltimore, Md.) · 2009Article
- Distinct responses of DREAM to electroacupuncture stimulation with different frequencies during physiological and inflammatory conditions in rats.Neurochemical research · 2008Article
- DREAM mediates cAMP-dependent, Ca2+-induced stimulation of GFAP gene expression and regulates cortical astrogliogenesis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- DREAM is reduced in synovial fibroblasts of patients with chronic arthritic pain: is it a suitable target for peripheral pain management?Arthritis research & therapy · 2008Article
- Distribution of downstream regulatory element antagonist modulator (DREAM) in rat spinal cord and upregulation of its expression during inflammatory pain.Neurochemical research · 2007Article
- Differential regulation of c-Fos and FosB in the rat brain after amygdala kindling.Cellular and molecular neurobiology · 2006Article
- Downstream regulatory element antagonist modulator regulates Ca2+ homeostasis and viability in cerebellar neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Transcriptional repressor DREAM regulates T-lymphocyte proliferation and cytokine gene expression.The EMBO journal · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Induction of the prodynorphin gene has been implicated in medium and long-term adaptation during memory acquisition and pain. By 5' deletion mapping and site-directed mutagenesis of the human prodynorphin promoter, we demonstrate that both basal transcription and protein kinase A (PKA)-induced transcription in NB69 and SK-N-MC human neuroblastoma cells are regulated by the GAGTCAAGG sequence centered at position +40 in the 5' untranslated region of the gene (named the DRE, for downstream regulatory element). The DRE repressed basal transcription in an orientation-independent and cell-specific manner when placed downstream from the heterologous thymidine kinase promoter. Southwestern blotting and UV cross-linking experiments with nuclear extracts from human neuroblastoma cells or human brain revealed a protein complex of approximately 110 kDa that specifically bound to the DRE. Forskolin treatment reduced binding to the DRE, and the time course paralleled that for an increase in prodynorphin gene expression. Our results suggest that under basal conditions, expression of the prodynorphin gene is repressed by occupancy of the DRE site. Upon PKA stimulation, binding to the DRE is reduced and transcription increases. We propose a model for human prodynorphin activation through PKA-dependent derepression at the DRE site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.