ArticleJournal of clinical microbiology1998
Evaluation of PCR for detection of DNA specific for Aspergillus species in sera of patients with various forms of pulmonary aspergillosis.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Clinical performance of a commercial real-time PCR assay for Aspergillus DNA detection in serum samples from high-risk patients: comparison with a galactomannan enzyme immunoassay.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Utility of PCR in diagnosis of invasive fungal infections: real-life data from a multicenter study.Journal of clinical microbiology · 2013Article
- Evaluation of analytical and preliminary clinical performance of Myconostica MycAssay Aspergillus when testing serum specimens for diagnosis of invasive Aspergillosis.Journal of clinical microbiology · 2011Article
- Comparison of non-culture-based methods for detection of systemic fungal infections, with an emphasis on invasive Candida infections.Journal of clinical microbiology · 2005Article
- Diagnosing invasive aspergillosis during antifungal therapy by PCR analysis of blood samples.Journal of clinical microbiology · 2004Article
- Current status of nonculture methods for diagnosis of invasive fungal infections.Clinical microbiology reviews · 2002Review
- Automated extraction of genomic DNA from medically important yeast species and filamentous fungi by using the MagNA Pure LC system.Journal of clinical microbiology · 2002Article
- Quantitative PCR assay to measure Aspergillus fumigatus burden in a murine model of disseminated aspergillosis: demonstration of efficacy of caspofungin acetate.Antimicrobial agents and chemotherapy · 2001Article
- Experimental model of progressive disseminated trichosporonosis in mice with latent trichosporonemia.Journal of clinical microbiology · 2000Article
- Identification of Aspergillus species using internal transcribed spacer regions 1 and 2.Journal of clinical microbiology · 2000Article
- PCR detection of DNA specific for Trichosporon species in serum of patients with disseminated trichosporonosis.Journal of clinical microbiology · 1999Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pulmonary aspergillosis is classified into invasive, saprophytic, and allergic forms. In this study, we evaluated the usefulness of PCR for differentiating between different forms of aspergillosis or in monitoring disease activity during treatment by detecting DNA specific for Aspergillus species in the serum. Nested PCR was used to detect Aspergillus DNA in the sera of 30 patients with various forms of pulmonary aspergillosis. The results were compared with those of latex agglutination tests for detecting galactomannan antigen. We also examined the serial changes in the results of nested PCR during and after treatment of a subgroup of patients with invasive pulmonary aspergillosis with amphotericin B. The highest proportion of positive nested PCR results were in patients with invasive aspergillosis (10 of 12; 83%), while patients with pulmonary aspergilloma had the lowest frequency of positive tests (1 of 9; 11%). These results suggested that the sensitivity of the nested PCR depends on the extent of invasion by Aspergillus species. Serial assays showed that the results of nested PCR became negative shortly after commencement of antifungal treatment and that such changes did not correlate with clinical responsiveness to treatment. Our results indicate the potential usefulness of nested PCR with serum samples for the diagnosis of invasive aspergillosis and the detection of a shift in the status of infection from a noninvasive type to invasive aspergillosis. However, the results of the nested PCR did not correlate with the response to antifungal treatment.
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