ArticleThe Journal of cell biology1998
Neuroendocrine synaptic vesicles are formed in vitro by both clathrin-dependent and clathrin-independent pathways.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 94 citations in OpenAlex.
- Role of adaptin protein complexes in intracellular trafficking and their impact on diseases.Bioengineered · 2021Article
- The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis.Molecular biology of the cell · 2013Article
- Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding.Methods in cell biology · 2012Article
- Vesicular zinc regulates the Ca2+ sensitivity of a subpopulation of presynaptic vesicles at hippocampal mossy fiber terminals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- An AP-3-dependent mechanism drives synaptic-like microvesicle biogenesis in pancreatic islet beta-cells.American journal of physiology. Endocrinology and metabolism · 2010Article
- CB(1) cannabinoid receptors and their associated proteins.Current medicinal chemistry · 2010Review
- Enhanced synaptic vesicle traffic in hippocampus of phenytoin-resistant kindled rats.Neurochemical research · 2009Article
- Synaptic vesicle protein trafficking at the glutamate synapse.Neuroscience · 2009Review
- Real-time imaging of discrete exocytic events mediating surface delivery of AMPA receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- BLOC-1 complex deficiency alters the targeting of adaptor protein complex-3 cargoes.Molecular biology of the cell · 2006Article
- Oligomerization and dissociation of AP-1 adaptors are regulated by cargo signals and by ArfGAP1-induced GTP hydrolysis.Molecular biology of the cell · 2005Article
- Phosphatidylinositol-4-kinase type II alpha is a component of adaptor protein-3-derived vesicles.Molecular biology of the cell · 2005Article
- Biochemical characterization of the coating mechanism of the endosomal donor compartment of synaptic vesicles.Neurochemical research · 2004Article
- The zinc transporter ZnT3 interacts with AP-3 and it is preferentially targeted to a distinct synaptic vesicle subpopulation.Molecular biology of the cell · 2004Article
- Signals involved in targeting membrane proteins to synaptic vesicles.Cellular and molecular neurobiology · 2002Review
- The beta-appendages of the four adaptor-protein (AP) complexes: structure and binding properties, and identification of sorting nexin 9 as an accessory protein to AP-2.The Biochemical journal · 2002Article
- Assembly and function of AP-3 complexes in cells expressing mutant subunits.The Journal of cell biology · 2002Article
- Rab4 regulates formation of synaptic-like microvesicles from early endosomes in PC12 cells.Molecular biology of the cell · 2001Article
- Identification of an axonal determinant in the C-terminus of the sodium channel Na(v)1.2.The EMBO journal · 2001Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
In the neuroendocrine cell line, PC12, synaptic vesicles can be generated from endosomes by a sorting and vesiculation process that requires the heterotetrameric adaptor protein AP3 and a small molecular weight GTPase of the ADP ribosylation factor (ARF) family. We have now discovered a second pathway that sorts the synaptic vesicle-associated membrane protein (VAMP) into similarly sized vesicles. For this pathway the plasma membrane is the precursor rather than endosomes. Both pathways require cytosol and ATP and are inhibited by GTPgammaS. The second pathway, however, uses AP2 instead of AP3 and is brefeldin A insensitive. The AP2-dependent pathway is inhibited by depletion of clathrin or by inhibitors of clathrin binding, whereas the AP3 pathway is not. The VAMP-containing, plasma membrane-derived vesicles can be readily separated on sucrose gradients from transferrin (Tf)-containing vesicles generated by incubating Tf-labeled plasma membrane preparations at 37 degreesC. Dynamin- interacting proteins are required for the AP2-mediated vesiculation from the plasma membrane, but not from endosomes. Thus, VAMP is sorted into small vesicles by AP3 and ARF1 at endosomes and by AP2 and clathrin at the plasma membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.