ArticleRNA (New York, N.Y.)1998
A pseudouridine synthase required for the formation of two universally conserved pseudouridines in ribosomal RNA is essential for normal growth of Escherichia coli.
Article in RNA (New York, N.Y.), 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed.
- Compendium of RNA modifications for bacterial stress adaptation.Microbiology and molecular biology reviews : MMBR · 2026Review
- Direct RNA Sequencing Reveals Stress-Dependent and Pathway-Specific rRNA Modification Reprogramming during 50S Biogenesis.Biochemistry · 2026Article
- Direct RNA Sequencing Reveals Stress-Dependent and Pathway-Specific rRNA Modification Reprogramming During 50S Biogenesis.bioRxiv : the preprint server for biology · 2026Article
- Suppressor analysis links trans-translation and ribosomal protein uS7 to RluD function in Escherichia coli.Biochemical and biophysical research communications · 2024Article
- RNA Post-Transcriptional Modifications in Two Large Subunit Intermediates Populated inBiochemistry · 2022Article
- Naturally occurring modified ribonucleosides.Wiley interdisciplinary reviews. RNA · 2020Review
- Structural and evolutionary insights into ribosomal RNA methylation.Nature chemical biology · 2018Review
- The Pseudouridine Synthases Proceed through a Glycal Intermediate.Journal of the American Chemical Society · 2016Article
- Identification of the methyltransferase targeting C2499 in Deinococcus radiodurans 23S ribosomal RNA.Extremophiles : life under extreme conditions · 2016Article
- Secreted multifunctional Glyceraldehyde-3-phosphate dehydrogenase sequesters lactoferrin and iron into cells via a non-canonical pathway.Scientific reports · 2015Article
- Structure modulation of helix 69 from Escherichia coli 23S ribosomal RNA by pseudouridylations.Nucleic acids research · 2014Article
- Pseudouridine: still mysterious, but never a fake (uridine)!RNA biology · 2014Review
- Indigenous and acquired modifications in the aminoglycoside binding sites of Pseudomonas aeruginosa rRNAs.RNA biology · 2013Article
- How much can we learn about the function of bacterial rRNA modification by mining large-scale experimental datasets?Nucleic acids research · 2012Article
- Role of pseudouridine in structural rearrangements of helix 69 during bacterial ribosome assembly.ACS chemical biology · 2012Article
- Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity.Nucleic acids research · 2012Article
- Transcriptional organization and physiological contributions of the relQ operon of Streptococcus mutans.Journal of bacteriology · 2012Article
- Mass spectrometry-based quantification of pseudouridine in RNA.Journal of the American Society for Mass Spectrometry · 2011Article
- A nano-chip-LC/MSn based strategy for characterization of modified nucleosides using reduced porous graphitic carbon as a stationary phase.Journal of the American Society for Mass Spectrometry · 2011Article
- Probing conformational states of modified helix 69 in 50S ribosomes.Journal of the American Chemical Society · 2011Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli rRNA contains 10 pseudouridines of unknown function. They are made by synthases, each of which is specific for one or more pseudouridines. Here we show that the sfhB (yfil) ORF of E. coli is a pseudouridine synthase gene by cloning, protein overexpression, and reaction in vitro with rRNA transcripts. Gene disruption by miniTn10(cam) insertion revealed that this synthase gene, here renamed rluD, codes for a synthase which is solely responsible in vivo for synthesis of the three pseudouridines clustered in a stem-loop at positions 1911, 1915, and 1917 of 23S RNA. The absence of RluD results in severe growth inhibition. Both the absence of pseudouridine and the growth defect could be reversed by insertion of a plasmid carrying the rluD gene into the mutant cell, clearly linking both effects to the absence of RIuD. This is the first report of a major physiological defect due to the deletion of any pseudouridine synthase. Growth inhibition may be due to the lack of one or more of the 23S RNA pseudouridines made by this synthase since pseudouridines 1915 and 1917 are universally conserved and are located in proximity to the decoding center of the ribosome where they could be involved in modulating codon recognition.
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