ArticleRNA (New York, N.Y.)1998
Inhibition of viral gene expression by human ribonuclease P.
Article in RNA (New York, N.Y.), 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 65 citations in OpenAlex.
- Review
- External Guide Sequence Effectively Suppresses the Gene Expression and Replication of Herpes Simplex Virus 2.Molecules (Basel, Switzerland) · 2024Article
- A RNase P Ribozyme Inhibits Gene Expression and Replication of Hepatitis B Virus in Cultured Cells.Microorganisms · 2023Article
- Mapping of RNase P Ribozyme Regions in Proximity with a Human RNase P Subunit Protein Using Fe(II)-EDTA Cleavage and Nuclease Footprint Analyses.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Inhibition of human cytomegalovirus major capsid protein expression and replication by ribonuclease P-associated external guide sequences.RNA (New York, N.Y.) · 2019Article
- Inhibition of Murine Cytomegalovirus Infection in Animals by RNase P-Associated External Guide Sequences.Molecular therapy. Nucleic acids · 2017Article
- Inhibition of herpes simplex virus 1 gene expression and replication by RNase P-associated external guide sequences.Scientific reports · 2016Article
- RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences.Biomolecules · 2015Review
- External guide sequence technology: a path to development of novel antimicrobial therapeutics.Annals of the New York Academy of Sciences · 2015Review
- Directing RNase P-mediated cleavage of target mRNAs by engineered external guide sequences in cultured cells.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Inhibition of hepatitis B virus gene expression and replication by ribonuclease P.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Article
- RNase P-associated external guide sequence effectively reduces the expression of human CC-chemokine receptor 5 and inhibits the infection of human immunodeficiency virus 1.BioMed research international · 2013Article
- Ribonuclease P-mediated inhibition of human cytomegalovirus gene expression and replication induced by engineered external guide sequences.RNA biology · 2012Article
- Effective inhibition of cytomegalovirus infection by external guide sequences in mice.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Evidence for recycling of external guide sequences during cleavage of bipartite substrates in vitro by reconstituted archaeal RNase P.Journal of molecular biology · 2011Article
- Engineered external guide sequences effectively block viral gene expression and replication in cultured cells.The Journal of biological chemistry · 2011Article
- Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P.Virology journal · 2010Article
- Impact of DNA gyrase inhibition by antisense ribozymes on rec A in E. coli.Molecular biology reports · 2009Article
- Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans.BMC biotechnology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
External guide sequences (EGSs) are small RNA molecules which consist of a sequence complementary to a target mRNA and render the target RNA susceptible to degradation by ribonuclease P (RNase P). EGSs were designed to target the mRNA encoding thymidine kinase (TK) of herpes simplex virus 1 for degradation. These EGSs were shown to be able to direct human RNase P to cleave the TK mRNA sequence efficiently in vitro. A reduction of about 80% in the expression level of both TK mRNA and protein was observed in human cells that steadily expressed an EGS, but not in cells that either did not express the EGS or produced a "disabled" EGS which carried a single nucleotide mutation that precluded RNase P recognition. Thus, EGSs may represent novel gene-targeting agents for inhibition of gene expression and antiviral activity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.