ArticleRNA (New York, N.Y.)1998
Target discrimination by RNA-binding proteins: role of the ancillary protein U2A' and a critical leucine residue in differentiating the RNA-binding specificity of spliceosomal proteins U1A and U2B".
Article in RNA (New York, N.Y.), 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- How does precursor RNA structure influence RNA processing and gene expression?Bioscience reports · 2023Review
- Phylogeny and conservation of plant U2A/U2A', a core splicing component in U2 spliceosomal complex.Planta · 2021Article
- A compare-and-contrast NMR dynamics study of two related RRMs: U1A and SNF.Biophysical journal · 2014Article
- Climbing the vertebrate branch of U1A/U2B″ protein evolution.RNA (New York, N.Y.) · 2014Article
- Binding affinity and cooperativity control U2B″/snRNA/U2A' RNP formation.Biochemistry · 2014Article
- Human U2B″ protein binding to snRNA stemloops.Biophysical chemistry · 2011Article
- Dimerization of FIR upon FUSE DNA binding suggests a mechanism of c-myc inhibition.The EMBO journal · 2008Article
- Recognition of essential purines by the U1A protein.BMC biochemistry · 2007Article
- Functional redundancy of worm spliceosomal proteins U1A and U2B''.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A study of collective atomic fluctuations and cooperativity in the U1A-RNA complex based on molecular dynamics simulations.Journal of structural biology · 2007Article
- Do collective atomic fluctuations account for cooperative effects? Molecular dynamics studies of the U1A-RNA complex.Journal of the American Chemical Society · 2006Article
- Substitution of an essential adenine in the U1A-RNA complex with a non-polar isostere.Nucleic acids research · 2002Article
- Investigation of a conserved stacking interaction in target site recognition by the U1A protein.Nucleic acids research · 2002Article
- T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from cDNA libraries.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- The Drosophila U2 snRNP protein U2A' has an essential function that is SNF/U2B" independent.Nucleic acids research · 2001Article
- Terminating eukaryote translation: domain 1 of release factor eRF1 functions in stop codon recognition.RNA (New York, N.Y.) · 2000Article
- Functional analysis of SNF, the Drosophila U1A/U2B" homolog: identification of dispensable and indispensable motifs for both snRNP assembly and function in vivo.RNA (New York, N.Y.) · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The spliceosomal proteins U1A and U2B" each use a homologous RRM domain to bind specifically to their respective snRNA targets, U1hpll and U2hpIV, two stem-loops that are similar yet distinct in sequence. Previous studies have shown that binding of U2B" to U2hpIV is facilitated by the ancillary protein U2A', whereas specific binding of U1A to U1hpll requires no cofactor. Here we report that U2A' enables U2B" to distinguish the loop sequence of U2hpIV from that of U1hpll but plays no role in stem sequence discrimination. Although U2A' can also promote heterospecific binding of U1A to U2hpIV, a much higher concentration of the ancillary protein is required due to the approximately 500-fold greater affinity of U2A' for U2B". Additional experiments have identified a single leucine residue in U1A(Leu-44) that is critical for the intrinsic specificity of this protein for the loop sequence of U1 hpll in preference to that of U2hpIV. Our data suggest that most of the difference in RNA-binding specificity between U1A and U2B" can be accounted for by this leucine residue and by the contribution of the ancillary protein U2A' to the specificity of U2B".
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.