ArticleThe Journal of cell biology1998
The yeast centrin, cdc31p, and the interacting protein kinase, Kic1p, are required for cell integrity.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 79 citations in OpenAlex.
- Kingdom-wide CRISPR guide design with ALLEGRO.Nucleic acids research · 2025Article
- Identification of Kic1p and Cdc42p as Novel Targets to Engineer Yeast Acetic Acid Stress Tolerance.Frontiers in bioengineering and biotechnology · 2022Article
- Structural Basis for the Functional Diversity of Centrins: A Focus on Calcium Sensing Properties and Target Recognition.International journal of molecular sciences · 2021Review
- TheCells · 2021Review
- The sperm centrioles.Molecular and cellular endocrinology · 2020Review
- Polarity in Ciliate Models: From Cilia to Cell Architecture.Frontiers in cell and developmental biology · 2019Review
- MST kinases in development and disease.The Journal of cell biology · 2015Review
- Mitotic exit and separation of mother and daughter cells.Genetics · 2012Review
- Such small hands: the roles of centrins/caltractins in the centriole and in genome maintenance.Cellular and molecular life sciences : CMLS · 2012Review
- Role of centrins 2 and 3 in organelle segregation and cytokinesis in Trypanosoma brucei.PloS one · 2012Article
- Quantitative proteomics of yeast post-Golgi vesicles reveals a discriminating role for Sro7p in protein secretion.Traffic (Copenhagen, Denmark) · 2011Article
- The yeast LATS/Ndr kinase Cbk1 regulates growth via Golgi-dependent glycosylation and secretion.Molecular biology of the cell · 2008Article
- The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability.Molecular biology of the cell · 2008Article
- Centrin/Cdc31 is a novel regulator of protein degradation.Molecular and cellular biology · 2008Article
- Characterization of differentiated quiescent and nonquiescent cells in yeast stationary-phase cultures.Molecular biology of the cell · 2008Article
- Centrin1 is required for organelle segregation and cytokinesis in Trypanosoma brucei.Molecular biology of the cell · 2007Article
- Mechanisms regulating the protein kinases of Saccharomyces cerevisiae.Eukaryotic cell · 2007Review
- Deletion of RNQ1 gene reveals novel functional relationship between divergently transcribed Bik1p/CLIP-170 and Sfi1p in spindle pole body separation.Current genetics · 2006Article
- Conserved elements of the RAM signaling pathway establish cell polarity in the basidiomycete Cryptococcus neoformans in a divergent fashion from other fungi.Molecular biology of the cell · 2006Article
- Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication.The Journal of cell biology · 2006Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cdc31p is the yeast homologue of centrin, a highly conserved calcium-binding protein of the calmodulin superfamily. Previously centrins have been implicated only in microtubule-based processes. To elucidate the functions of yeast centrin, we carried out a two-hybrid screen for Cdc31p-interacting proteins and identified a novel essential protein kinase of 1,080 residues, Kic1p (kinase that interacts with Cdc31p). Kic1p is closely related to S. cerevisiae Ste20p and the p-21- activated kinases (PAKs) found in a wide variety of eukaryotic organisms. Cdc31p physically interacts with Kic1p by two criteria; Cdc31p coprecipitated with GST-Kic1p and it bound to GST-Kic1p in gel overlay assays. Furthermore, GST-Kic1p exhibited in vitro kinase activity that was CDC31-dependent. Although kic1 mutants were not defective for spindle pole body duplication, they exhibited a variety of mutant phenotypes demonstrating that Kic1p is required for cell integrity. We also found that cdc31 mutants, previously identified as defective for spindle pole body duplication, exhibited lysis and morphological defects. The cdc31 kic1 double mutants exhibited a drastic reduction in the range of permissive temperature, resulting in a severe lysis defect. We conclude that Kic1p function is dependent upon Cdc31p both in vivo and in vitro. We postulate that Cdc31p is required both for SPB duplication and for cell integrity/morphogenesis, and that the integrity/morphogenesis function is mediated through the Kic1p protein kinase.
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