ArticleProceedings of the National Academy of Sciences of the United States of America1998
Reciprocal subtraction differential RNA display: an efficient and rapid procedure for isolating differentially expressed gene sequences.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Benzo[a]pyrene increases the Nrf2 content by downregulating the Keap1 message.Toxicological sciences : an official journal of the Society of Toxicology · 2010Article
- Cloning and characterization of SARI (suppressor of AP-1, regulated by IFN).Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Differential profiling of selected defence-related genes induced on challenge with Alternaria brassicicola in resistant white mustard and their comparative expression pattern in susceptible India mustard.Molecular plant pathology · 2008Article
- Impact of light on Hypocrea jecorina and the multiple cellular roles of ENVOY in this process.BMC genomics · 2007Article
- Melanoma differentiation associated gene-7/interleukin-24 (mda-7/IL-24): novel gene therapeutic for metastatic melanoma.Toxicology and applied pharmacology · 2007Review
- Astrocyte elevated gene-1: recent insights into a novel gene involved in tumor progression, metastasis and neurodegeneration.Pharmacology & therapeutics · 2007Review
- Subtractive differential display: a modified differential display technique for isolating differentially expressed genes.Molecular biology reports · 2007Article
- mda-7/IL-24: multifunctional cancer-specific apoptosis-inducing cytokine.Pharmacology & therapeutics · 2006Review
- ooc1, a unique gene expressed only during growth of Hypocrea jecorina (anamorph: Trichoderma reesei) on cellulose.Current genetics · 2005Article
- Identification of calcium- and nitric oxide-regulated genes by differential analysis of library expression (DAzLE).Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Identification of gene products suppressed by human immunodeficiency virus type 1 infection or gp120 exposure of primary human astrocytes by rapid subtraction hybridization.Journal of neurovirology · 2003Article
- mda-7 (IL-24) Mediates selective apoptosis in human melanoma cells by inducing the coordinated overexpression of the GADD family of genes by means of p38 MAPK.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
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- A critical evaluation of differential display as a tool to identify genes involved in legume nodulation: looking back and looking forward.Nucleic acids research · 2001Article
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- PEA3 sites within the progression elevated gene-3 (PEG-3) promoter and mitogen-activated protein kinase contribute to differential PEG-3 expression in Ha-ras and v-raf oncogene transformed rat embryo cells.Nucleic acids research · 2001Article
- RaSH, a rapid subtraction hybridization approach for identifying and cloning differentially expressed genes.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
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4 authors.
Funding
Abstract
A reciprocal subtraction differential RNA display (RSDD) approach has been developed that permits the rapid and efficient identification and cloning of both abundant and rare differentially expressed genes. RSDD comprises reciprocal subtraction of cDNA libraries followed by differential RNA display. The RSDD strategy was applied to analyze the gene expression alterations resulting during cancer progression as adenovirus-transformed rodent cells developed an aggressive transformed state, as documented by elevated anchorage-independence and enhanced in vivo oncogenesis in nude mice. This approach resulted in the identification and cloning of both known and a high proportion (>65%) of unknown sequences, including cDNAs displaying elevated expression as a function of progression (progression-elevated gene) and cDNAs displaying suppressed expression as a function of progression (progression-suppressed gene). Sixteen differentially expressed genes, including five unknown progression-elevated genes and six unknown progression-suppressed genes, have been characterized. The RSDD scheme should find wide application for the effective detection and isolation of differentially expressed genes.
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