ArticleProceedings of the National Academy of Sciences of the United States of America1998
ADP-ribosylation factor and phosphatidic acid levels in Golgi membranes during budding of coatomer-coated vesicles.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 46 citations in OpenAlex.
- Membrane Curvature, Trans-Membrane Area Asymmetry, Budding, Fission and Organelle Geometry.International journal of molecular sciences · 2020Review
- Models of Intracellular Transport: Pros and Cons.Frontiers in cell and developmental biology · 2019Article
- Legionella pneumophila Effector LpdA Is a Palmitoylated Phospholipase D Virulence Factor.Infection and immunity · 2015Article
- ARF1-regulated coatomer directs the steady-state localization of protein kinase C epsilon at the Golgi apparatus.Biochimica et biophysica acta · 2013Article
- A role for phosphatidic acid in COPI vesicle fission yields insights into Golgi maintenance.Nature cell biology · 2008Article
- Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor.Journal of virology · 2005Article
- Phosphatidylinositol 4,5-bisphosphate and Arf6-regulated membrane traffic.The Journal of cell biology · 2001Article
- Intracellular localization of phospholipase D1 in mammalian cells.Molecular biology of the cell · 2001Article
- Role for phospholipase D in receptor-mediated endocytosis.Molecular and cellular biology · 2001Article
- Phosphoinositide-AP-2 interactions required for targeting to plasma membrane clathrin-coated pits.The Journal of cell biology · 1999Article
- ADP ribosylation factor 1 mutants identify a phospholipase D effector region and reveal that phospholipase D participates in lysosomal secretion but is not sufficient for recruitment of coatomer I.The Biochemical journal · 1999Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The finding that ADP-ribosylation factor (ARF) can activate phospholipase D has led to debate as to whether ARF recruits coat proteins through direct binding or indirectly by catalytically increasing phosphatidic acid production. Here we test critical aspects of these hypotheses. We find that Golgi membrane phosphatidic acid levels do not rise-in fact they decline-during cell-free budding reactions. We confirm that the level of membrane-bound ARF can be substantially reduced without compromising coat assembly [Ktistakis, N. T., Brown, H. A., Waters, M. G., Sternweis, P. C. & Roth, M. G. (1996) J. Cell Biol. 134, 295-306], but find that under all conditions, ARF is present on the Golgi membrane in molar excess over bound coatomer. These results do not support the possibility that the activation of coat assembly by ARF is purely catalytic, and they are consistent with ARF forming direct interactions with coatomer. We suggest that ARF, like many other G proteins, is a multifunctional protein with roles in trafficking and phospholipid signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.