ArticleJournal of virology1998
The LEF-4 subunit of baculovirus RNA polymerase has RNA 5'-triphosphatase and ATPase activities.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 90 citations in OpenAlex.
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- Comparative genomics of the Neodiprion sertifer nucleopolyhedrovirus from Turkey with the fewest ORFs among baculoviruses.Virus genes · 2024Article
- A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells.Protein expression and purification · 2021Review
- Responses of the Housefly,Frontiers in microbiology · 2017Article
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- Mutualistic polydnaviruses share essential replication gene functions with pathogenic ancestors.PLoS pathogens · 2013Article
- Analysis of the genome of the sexually transmitted insect virus Helicoverpa zea nudivirus 2.Viruses · 2012Article
- Baculovirus: molecular insights on their diversity and conservation.International journal of evolutionary biology · 2011Article
- Characterization of LEF4 ligand binding property and its role as part of baculoviral transcription machinery.Molecular and cellular biochemistry · 2010Article
- Polyphosphatase activity of CthTTM, a bacterial triphosphate tunnel metalloenzyme.The Journal of biological chemistry · 2008Article
- Roles of LEF-4 and PTP/BVP RNA triphosphatases in processing of baculovirus late mRNAs.Journal of virology · 2008Article
- Genome sequence of Leucania seperata nucleopolyhedrovirus.Virus genes · 2007Article
- Structure-function analysis of Plasmodium RNA triphosphatase and description of a triphosphate tunnel metalloenzyme superfamily that includes Cet1-like RNA triphosphatases and CYTH proteins.RNA (New York, N.Y.) · 2006Article
- Expression of baculovirus late and very late genes depends on LEF-4, a component of the viral RNA polymerase whose guanyltransferase function is essential.Journal of virology · 2006Article
- Mapping the triphosphatase active site of baculovirus mRNA capping enzyme LEF4 and evidence for a two-metal mechanism.Nucleic acids research · 2003Article
- Autographa californica nucleopolyhedrovirus orf69 encodes an RNA cap (nucleoside-2'-O)-methyltransferase.Journal of virology · 2003Article
- Functional characterization and structural modelling of late gene expression factor 4 from Bombyx mori nucleopolyhedrovirus.The Biochemical journal · 2002Article
- What messenger RNA capping tells us about eukaryotic evolution.Nature reviews. Molecular cell biology · 2002Review
- Analysis of an Autographa californica multicapsid nucleopolyhedrovirus lef-6-null virus: LEF-6 is not essential for viral replication but appears to accelerate late gene transcription.Journal of virology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The baculovirus Autographa californica nuclear polyhedrosis virus encodes a DNA-dependent RNA polymerase that is required for transcription of viral late genes. This polymerase is composed of four equimolar subunits, LEF-8, LEF-4, LEF-9, and p47. The LEF-4 subunit has guanylyltransferase activity, suggesting that baculoviruses may encode a full complement of capping enzymes. Here we show that LEF-4 is a bifunctional enzyme that hydrolyzes the gamma phosphates of triphosphate-terminated RNA and also hydrolyzes ATP and GTP to the respective diphosphate forms. Alanine substitution of five residues previously shown to be essential for vaccinia virus RNA triphosphatase activity inactivated the triphosphatase component of LEF-4 but not the guanylyltransferase domain. Conversely, mutation of the invariant lysine in the guanylyltransferase domain abolished the guanylyltransferase activity without affecting triphosphatase function. We also investigated the effects of substituting phenylalanine for leucine at position 105, a mutation that results in a virus that is temperature sensitive for late gene expression. We found that this mutation had no significant effect on the ATPase or guanylyltransferase activity of LEF-4 but resulted in a modest decrease in RNA triphosphatase activity.
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