ArticleJournal of virology1998
Guanylyltransferase activity of the LEF-4 subunit of baculovirus RNA polymerase.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 62 citations in OpenAlex.
- Enzymatic Assays to Explore Viral mRNA Capping Machinery.Chembiochem : a European journal of chemical biology · 2021Review
- Baculovirus FP25K Localization: Role of the Coiled-Coil Domain.Journal of virology · 2016Article
- Article
- Enhanced protein secretion from insect cells by co-expression of the chaperone calreticulin and translation initiation factor eIF4E.Molecular biotechnology · 2013Article
- Baculovirus: molecular insights on their diversity and conservation.International journal of evolutionary biology · 2011Article
- A dual interface determines the recognition of RNA polymerase II by RNA capping enzyme.The Journal of biological chemistry · 2010Article
- Characterization of LEF4 ligand binding property and its role as part of baculoviral transcription machinery.Molecular and cellular biochemistry · 2010Article
- Roles of LEF-4 and PTP/BVP RNA triphosphatases in processing of baculovirus late mRNAs.Journal of virology · 2008Article
- Molecular characterization and genetic organization of the inhibitor of apoptosis gene (iap-5) region of the Pieris rapae granulovirus.Virus genes · 2007Article
- Expression of baculovirus late and very late genes depends on LEF-4, a component of the viral RNA polymerase whose guanyltransferase function is essential.Journal of virology · 2006Article
- Autographa californica nucleopolyhedrovirus orf69 encodes an RNA cap (nucleoside-2'-O)-methyltransferase.Journal of virology · 2003Article
- Mapping the triphosphatase active site of baculovirus mRNA capping enzyme LEF4 and evidence for a two-metal mechanism.Nucleic acids research · 2003Article
- Functional characterization and structural modelling of late gene expression factor 4 from Bombyx mori nucleopolyhedrovirus.The Biochemical journal · 2002Article
- What messenger RNA capping tells us about eukaryotic evolution.Nature reviews. Molecular cell biology · 2002Review
- Analysis of an Autographa californica multicapsid nucleopolyhedrovirus lef-6-null virus: LEF-6 is not essential for viral replication but appears to accelerate late gene transcription.Journal of virology · 2002Article
- RNA triphosphatase component of the mRNA capping apparatus of Paramecium bursaria Chlorella virus 1.Journal of virology · 2001Article
- 3'-end formation of baculovirus late RNAs.Journal of virology · 2000Article
- A yeast-based genetic system for functional analysis of viral mRNA capping enzymes.Journal of virology · 2000Article
- Viral and cellular mRNA capping: past and prospects.Advances in virus research · 2000Review
- RNA 5'-triphosphatase, nucleoside triphosphatase, and guanylyltransferase activities of baculovirus LEF-4 protein.Journal of virology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The baculovirus Autographa californica nuclear polyhedrosis virus encodes a DNA-dependent RNA polymerase that transcribes viral late genes. This polymerase is composed of four equimolar subunits, LEF-4, LEF-8, LEF-9, and p47. Here we present data indicating that the LEF-4 subunit of RNA polymerase is a guanylyltransferase. Incubation of RNA polymerase in the presence of divalent cation and radiolabeled GTP resulted in the formation of a covalent enzyme-guanylate complex that comigrated with the LEF-4 subunit. The label transfer assay showed an absolute requirement for divalent cation which could be satisfied by either manganese or magnesium. The reaction was specific for guanine nucleotides, and GTP was more effective than dGTP in the formation of enzyme-guanylate complex. To demonstrate that LEF-4 was the guanylyltransferase, the single subunit was overexpressed in baculovirus-infected cells. The overexpressed protein was primarily cytosolic, indicating that other proteins in the RNA polymerase complex were responsible for nuclear targeting of LEF-4. LEF-4 alone was able to covalently bind GMP, although less efficiently than viral RNA polymerase.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.