ArticleJournal of virology1998
Influence of cell polarity on retrovirus-mediated gene transfer to differentiated human airway epithelia.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 95 citations in OpenAlex.
- High ionic strength vector formulations enhance gene transfer to airway epithelia.Nucleic acids research · 2024Article
- High ionic strength vector formulations enhance gene transfer to airway epithelia.bioRxiv : the preprint server for biology · 2024Article
- Sendai F/HN pseudotyped lentiviral vector transduces human ciliated and non-ciliated airway cells using α 2,3 sialylated receptors.Molecular therapy. Methods & clinical development · 2022Article
- Progress in Respiratory Gene Therapy.Human gene therapy · 2022Review
- New Directions in Pulmonary Gene Therapy.Human gene therapy · 2020Review
- Review
- Review
- Article
- Hybrid nonviral/viral vector systems for improved piggyBac DNA transposon in vivo delivery.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
- Response of Differentiated Human Airway Epithelia to Alcohol Exposure andMedical sciences (Basel, Switzerland) · 2013Article
- Gene delivery to the airway.Current protocols in human genetics · 2013Review
- Differentiated swine airway epithelial cell cultures for the investigation of influenza A virus infection and replication.Influenza and other respiratory viruses · 2013Article
- Manipulation of Cell Physiology Enables Gene Silencing in Well-differentiated Airway Epithelia.Molecular therapy. Nucleic acids · 2012Article
- Interferon-β sensitivity of tumor cells correlates with poor response to VA7 virotherapy in mouse glioma models.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- Hoechst increases adeno-associated virus-mediated transgene expression in airway epithelia by inducing the cytomegalovirus promoter.The journal of gene medicine · 2012Article
- Direct and indirect roles for β-catenin in facultative basal progenitor cell differentiation.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- Suppression of adenosine-activated chloride transport by ethanol in airway epithelia.PloS one · 2012Article
- Sites in the AAV5 capsid tolerant to deletions and tandem duplications.Archives of biochemistry and biophysics · 2010Article
- Lentiviral vectors and cystic fibrosis gene therapy.Viruses · 2010Article
- Impact of lentiviral vector-mediated transduction on the tightness of a polarized model of airway epithelium and effect of cationic polymer polyethylenimine.Journal of biomedicine & biotechnology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Gene transfer with recombinant murine leukemia viruses (MuLV) provides the potential to permanently correct inherited lung diseases, such as cystic fibrosis (CF). Several problems prevent the application of MuLV-based recombinant retroviruses to lung gene therapy: (i) the lack of cell proliferation in mature pulmonary epithelia, (ii) inefficient gene transfer with a vector applied to the apical surface, and (iii) low titers of many retroviral preparations. We found that keratinocyte growth factor (KGF) stimulated proliferation of differentiated human tracheal and bronchial epithelia. Approximately 50% of epithelia divided in response to KGF as assessed by bromodeoxyuridine histochemistry. In airway epithelia stimulated to divide with KGF, high-titer ampho- and xenotropic enveloped vectors preferentially infected cells from the basal side. However, treatment with hypotonic shock or EGTA transiently increased transepithelial permeability, enhancing gene transfer with the vector applied to the mucosal surfaces of KGF-stimulated epithelia. Up to 35% of cells expressed the transgene after gene transfer. By using this approach, cells throughout the epithelial sheet, including basal cells, were targeted. Moreover, the Cl- transport defect in differentiated CF airway epithelia was corrected. These findings suggest that barriers to apical infection with MuLV can be overcome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.