ArticleThe American journal of pathology1998
Interferon-gamma-secreting T-cell populations in rejecting murine cardiac allografts: assessment by flow cytometry.
Article in The American journal of pathology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed.
- High-resolution phenotyping of early acute rejection reveals a conserved alloimmune signature.Cell reports · 2021Article
- CD96 expression determines the inflammatory potential of IL-9-producing Th9 cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The Transcription Factor NFATc1 Supports the Rejection of Heterotopic Heart Allografts.Frontiers in immunology · 2018Article
- Role of NK, NKT cells and macrophages in liver transplantation.World journal of gastroenterology · 2016Review
- Differential expression of microRNAs during allograft rejection.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2012Article
- An age-specific CD8+ T cell pathway that impairs the effectiveness of strategies to prolong allograft survival.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- CXCR3 ligands contribute to Th1-induced inflammation but not to homing of Th1 cells into the lung.Experimental lung research · 2008Article
- IFN-gamma, produced by NK cells that infiltrate liver allografts early after transplantation, links the innate and adaptive immune responses.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2005Article
- Quantitative analysis of myocardial inflammation by flow cytometry in murine autoimmune myocarditis: correlation with cardiac function.The American journal of pathology · 2004Article
- Differential role for competitive reverse transcriptase-polymerase chain reaction and intracellular cytokine staining as diagnostic tools for the assessment of intragraft cytokine profiles in rejecting and nonrejecting heart allografts.The American journal of pathology · 2000Article
- Association of B7-1 co-stimulation with the development of graft arterial disease. Studies using mice lacking B7-1, B7-2, or B7-1/B7-2.The American journal of pathology · 2000Article
- Interleukin-10 (IL-10) augments allograft arterial disease: paradoxical effects of IL-10 in vivo.The American journal of pathology · 1999Article
- Th1/Th2 balance in cancer, transplantation and pregnancy.Springer seminars in immunopathology · 1999Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Interplay between T-helper-1 (Th1) and T-helper-2 (Th2) cells is considered important in the development of acute allograft rejection and many other immune-mediated disease processes. Existing methods for evaluating expression of Th1 and Th2 cytokines, including reverse transcriptase polymerase chain reaction (RT-PCR), RNase protection assay (RPA), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) all have limitations; alternate techniques to quantify cell populations expressing specific cytokine proteins, generate statistically analyzable data, and allow simultaneous identification of cytokine-secreting cell type are needed. To this end, we adapted a flow cytometric technique for intracellular cytokine immunofluorescence staining for use with cells isolated from solid tissue. To demonstrate the utility of the method, we determined the number of CD4+ and CD8+ cells secreting the prototypical Th1 and Th2 cytokines, interferon (IFN)-gamma, and interleukin (IL)-4 in acutely rejecting murine cardiac allografts. We also measured the cytokine production via ELISA, RPA, and semiquantitative competitive RT-PCR. The number of CD4+ cells producing IFN-gamma increased as rejection proceeded, in agreement with previous data; we detected no IL-4 production at any time, although relatively low numbers of IL-10-producing cells were identified. In addition, a high percentage of CD8+ cells, which outnumber CD4+ cells at day 6 after transplant, also produce IFN-gamma, suggesting that cytotoxic lymphocytes contribute significantly to the local cytokine milieu. This new application of intracellular cytokine staining provides a powerful methodology for studying transplantation immunology. The method may also be easily adapted to the study of other immune-mediated processes.
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