ArticlePlant physiology1998
Plasma membrane-associated actin in bright yellow 2 tobacco cells. Evidence for interaction with microtubules
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 60 citations in OpenAlex.
- Actin filaments regulate the adhesion between the plasma membrane and the cell wall of tobacco guard cells.Protoplasma · 2018Article
- Gravity sensing, a largely misunderstood trigger of plant orientated growth.Frontiers in plant science · 2014Review
- Inhibitors of myosin, but not actin, alter transport through Tradescantia plasmodesmata.Protoplasma · 2011Article
- The type II Arabidopsis formin14 interacts with microtubules and microfilaments to regulate cell division.The Plant cell · 2010Article
- Microtubules are a target for self-incompatibility signaling in Papaver pollen.Plant physiology · 2008Article
- Microtubule-associated proteins in higher plants.Journal of plant research · 2007Review
- ARP2 and ARP3 are localized to sites of actin filament nucleation in tobacco BY-2 cells.Protoplasma · 2006Article
- The peroxisomal multifunctional protein interacts with cortical microtubules in plant cells.BMC cell biology · 2005Article
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- Reorganization and in vivo dynamics of microtubules during Arabidopsis root hair development.Plant physiology · 2004Article
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- Endoplasmic microtubules configure the subapical cytoplasm and are required for fast growth of Medicago truncatula root hairs.Plant physiology · 2002Article
- Plant nuclei can contain extensive grooves and invaginations.The Plant cell · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasma membrane ghosts form when plant protoplasts attached to a substrate are lysed to leave a small patch of plasma membrane. We have identified several factors, including the use of a mildly acidic actin stabilization buffer and the inclusion of glutaraldehyde in the fixative, that allow immunofluorescent visualization of extensive cortical actin arrays retained on membrane ghosts made from tobacco (Nicotiana tabacum L.) suspension-cultured cells (line Bright Yellow 2). Normal microtubule arrays were also retained using these conditions. Membrane-associated actin is random; it exhibits only limited coalignment with the microtubules, and microtubule depolymerization in whole cells before wall digestion and ghost formation has little effect on actin retention. Actin and microtubules also exhibit different sensitivities to the pH and K+ and Ca2+ concentrations of the lysis buffer. There is, however, strong evidence for interactions between actin and the microtubules at or near the plasma membrane, because both ghosts and protoplasts prepared from taxol-pretreated cells have microtubules arranged in parallel arrays and an increased amount of actin coaligned with the microtubules. These experiments suggest that the organization of the cortical actin arrays may be dependent on the localization and organization of the microtubules.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.