ArticlePlant physiology1998
A high-affinity Ca2+ pump, ECA1, from the endoplasmic reticulum is inhibited by cyclopiazonic acid but not by thapsigargin.
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 77 citations in OpenAlex.
- An intracellular CPK-ECA1 phosphoregulatory circuit couples calcium signatures to ABA homeostasis for plant osmosensivity.Science advances · 2025Article
- Evolutionary and Regulatory Pattern Analysis of Soybean CaFrontiers in plant science · 2022Article
- Simultaneous imaging of ER and cytosolic Ca2+ dynamics reveals long-distance ER Ca2+ waves in plants.Plant physiology · 2021Article
- MIZ1 regulates ECA1 to generate a slow, long-distance phloem-transmitted CaProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Calcium dynamics in tomato pollen tubes using the Yellow Cameleon 3.6 sensor.Plant reproduction · 2018Article
- Pharmacological Strategies for Manipulating Plant CaInternational journal of molecular sciences · 2018Review
- Genome-wide analysis of wheat calcium ATPases and potential role of selected ACAs and ECAs in calcium stress.BMC plant biology · 2017Article
- Ca(2+) monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor.Scientific reports · 2016Article
- Analyses of Ca2+ accumulation and dynamics in the endoplasmic reticulum of Arabidopsis root cells using a genetically encoded Cameleon sensor.Plant physiology · 2013Article
- ATP protects against FITC labeling of Solanum lycopersicon and Arabidopsis thaliana Ca2+-ATPase ATP binding domains.Plant physiology and biochemistry : PPB · 2013Article
- ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis.Nature communications · 2012Article
- Fine-tuning of the cytoplasmic Ca2+ concentration is essential for pollen tube growth.Plant physiology · 2009Article
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- A distinct endosomal Ca2+/Mn2+ pump affects root growth through the secretory process.Plant physiology · 2008Article
- ECA3, a Golgi-localized P2A-type ATPase, plays a crucial role in manganese nutrition in Arabidopsis.Plant physiology · 2008Article
- Cell death suppressor Arabidopsis bax inhibitor-1 is associated with calmodulin binding and ion homeostasis.Plant physiology · 2007Article
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- An endoplasmic reticulum-bound Ca(2+)/Mn(2+) pump, ECA1, supports plant growth and confers tolerance to Mn(2+) stress.Plant physiology · 2002Article
- A thapsigargin-sensitive Ca(2+) pump is present in the pea Golgi apparatus membrane.Plant physiology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify and characterize individual Ca2+ pumps, we have expressed an Arabidopsis ECA1 gene encoding an endoplasmic reticulum-type Ca2+-ATPase homolog in the yeast (Saccharomyces cerevisiae) mutant K616. The mutant (pmc1pmr1cnb1) lacks a Golgi and a vacuolar membrane Ca2+ pump and grows very poorly on Ca2+-depleted medium. Membranes isolated from the mutant showed high H+/Ca2+-antiport but no Ca2+-pump activity. Expression of ECA1 in endomembranes increased mutant growth by 10- to 20-fold in Ca2+-depleted medium. 45Ca2+ pumping into vesicles from ECA1 transformants was detected after the H+/Ca2+-antiport activity was eliminated with bafilomycin A1 and gramicidin D. The pump had a high affinity for Ca2+ (Km = 30 nM) and displayed two affinities for ATP (Km of 20 and 235 microM). Cyclopiazonic acid, a specific blocker of animal sarcoplasmic/endoplasmic reticulum Ca2+-ATPase, inhibited Ca2+ transport (50% inhibition dose = 3 nmol/mg protein), but thapsigargin (3 microM) did not. Transport was insensitive to calmodulin. These results suggest that this endoplasmic reticulum-type Ca2+-ATPase could support cell growth in plants as in yeast by maintaining submicromolar levels of cytosolic Ca2+ and replenishing Ca2+ in endomembrane compartments. This study demonstrates that the yeast K616 mutant provides a powerful expression system to study the structure/function relationships of Ca2+ pumps from eukaryotes.
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