ArticleThe Journal of general physiology1998
Mechanism of peptide-induced mast cell degranulation. Translocation and patch-clamp studies.
Article in The Journal of general physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Mastoparans: A Group of Multifunctional α-Helical Peptides With Promising Therapeutic Properties.Frontiers in molecular biosciences · 2022Review
- Neuroimmune Mechanisms in Signaling of Pain During Acute Kidney Injury (AKI).Frontiers in medicine · 2020Review
- Article
- MrgX2‑mediated internalization of LL‑37 and degranulation of human LAD2 mast cells.Molecular medicine reports · 2018Article
- Neuro-immune interactions in allergic diseases: novel targets for therapeutics.International immunology · 2017Article
- Roles of Mas-related G protein-coupled receptor X2 on mast cell-mediated host defense, pseudoallergic drug reactions, and chronic inflammatory diseases.The Journal of allergy and clinical immunology · 2016Review
- Submillisecond Dynamics of Mastoparan X Insertion into Lipid Membranes.The journal of physical chemistry letters · 2016Article
- TRPM7 kinase activity regulates murine mast cell degranulation.The Journal of physiology · 2016Article
- Protease-activated receptors in the Achilles tendon-a potential explanation for the excessive pain signalling in tendinopathy.Molecular pain · 2015Article
- Tachykinin peptide, substance P, and its receptor NK-1R play an important role in alimentary tract mucosal inflammation during cytotoxic therapy.Digestive diseases and sciences · 2014Review
- Substance P in heart failure: the good and the bad.International journal of cardiology · 2014Review
- Direct and indirect antimicrobial activities of neuropeptides and their therapeutic potential.Current protein & peptide science · 2012Review
- Non-FcεR bearing mast cells secrete sufficient interleukin-4 to control Francisella tularensis replication within macrophages.Cytokine · 2011Article
- Effects of endogenous substance P expression on degranulation in RBL-2H3 cells.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2011Article
- Interaction between sensory C-fibers and cardiac mast cells in ischemia/reperfusion: activation of a local renin-angiotensin system culminating in severe arrhythmic dysfunction.The Journal of pharmacology and experimental therapeutics · 2010Article
- End-tagging of ultra-short antimicrobial peptides by W/F stretches to facilitate bacterial killing.PloS one · 2009Article
- RDP58 inhibits T cell-mediated bladder inflammation in an autoimmune cystitis model.Journal of autoimmunity · 2008Article
- Article
- Adrenomedullin is a cross-talk molecule that regulates tumor and mast cell function during human carcinogenesis.The American journal of pathology · 2006Article
- Cyclic AMP is sufficient for triggering the exocytic recruitment of aquaporin-2 in renal epithelial cells.EMBO reports · 2003Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Substance P and other polycationic peptides are thought to stimulate mast cell degranulation via direct activation of G proteins. We investigated the ability of extracellularly applied substance P to translocate into mast cells and the ability of intracellularly applied substance P to stimulate degranulation. In addition, we studied by reverse transcription--PCR whether substance P-specific receptors are present in the mast cell membrane. To study translocation, a biologically active and enzymatically stable fluorescent analogue of substance P was synthesized. A rapid, substance P receptor- and energy-independent uptake of this peptide into pertussis toxin-treated and -untreated mast cells was demonstrated using confocal laser scanning microscopy. The peptide was shown to localize preferentially on or inside the mast cell granules using electron microscopic autoradiography with 125I-labeled all-D substance P and 3H-labeled substance P. Cell membrane capacitance measurements using the patch-clamp technique demonstrated that intracellularly applied substance P induced calcium transients and activated mast cell exocytosis with a time delay that depended on peptide concentration (delay of 100-500 s at concentrations of substance P from 50 to 5 microM). Degranulation in response to intracellularly applied substance P was inhibited by GDPbetaS and pertussis toxin, suggesting that substance P acts via G protein activation. These results support the recently proposed model of a receptor-independent mechanism of peptide-induced mast cell degranulation, which assumes a direct interaction of peptides with G protein alpha subunits subsequent to their translocation across the plasma membrane.
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