ArticleMolecular biology of the cell1998
Contribution of the GTPase domain to the subcellular localization of dynamin in the nematode Caenorhabditis elegans.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Developmental basis for intestinal barrier against the toxicity of graphene oxide.Particle and fibre toxicology · 2018Article
- The evolution of dynamin to regulate clathrin-mediated endocytosis: speculations on the evolutionarily late appearance of dynamin relative to clathrin-mediated endocytosis.BioEssays : news and reviews in molecular, cellular and developmental biology · 2012Article
- Defective membrane remodeling in neuromuscular diseases: insights from animal models.PLoS genetics · 2012Review
- A monoclonal antibody toolkit for C. elegans.PloS one · 2010Article
- Live-cell imaging in Caenorhabditis elegans reveals the distinct roles of dynamin self-assembly and guanosine triphosphate hydrolysis in the removal of apoptotic cells.Molecular biology of the cell · 2010Article
- Identification and partial characterization of a dynamin-like protein, EhDLP1, from the protist parasite Entamoeba histolytica.Eukaryotic cell · 2010Article
- Clathrin adaptor AP-1 complex excludes multiple postsynaptic receptors from axons in C. elegans.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Cellular and molecular determinants targeting the Caenorhabditis elegans PHR protein RPM-1 to perisynaptic regions.Developmental dynamics : an official publication of the American Association of Anatomists · 2008Article
- Apoptosis and aging in mitochondrial morphology mutants of S. cerevisiae.Folia microbiologica · 2007Article
- UNC-13 and UNC-10/rim localize synaptic vesicles to specific membrane domains.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Mutations in Caenorhabditis elegans cytoplasmic dynein components reveal specificity of neuronal retrograde cargo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- A dynamin-like protein (ADL2b), rather than FtsZ, is involved in Arabidopsis mitochondrial division.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A conserved mechanism of synaptogyrin localization.Molecular biology of the cell · 2001Article
- Dynamic visualization of nervous system in live Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Caenorhabditis elegans dynamin is expressed at high levels in neurons and at lower levels in other cell types, consistent with the important role that dynamin plays in the recycling of synaptic vesicles. Indirect immunofluorescence showed that dynamin is concentrated along the dorsal and ventral nerve cords and in the synapse-rich nerve ring. Green fluorescent protein (GFP) fused to the N terminus of dynamin is localized to synapse-rich regions. Furthermore, this chimera was detected along the apical membrane of intestinal cells, in spermathecae, and in coelomocytes. Dynamin localization was not affected by disrupting axonal transport of synaptic vesicles in the unc-104 (kinesin) mutant. To investigate the alternative mechanisms that dynamin might use for translocation to the synapse, we systematically tested the localization of different protein domains by fusion to GFP. Localization of each chimera was measured in one specific neuron, the ALM. The GTPase, a middle domain, and the putative coiled coil each contribute to synaptic localization. Surprisingly, the pleckstrin homology domain and the proline-rich domain, which are known to bind to coated-pit constituents, did not contribute to synaptic localization. The GFP-GTPase chimera was most strongly localized, although the GTPase domain has no known interactions with proteins other than with dynamin itself. Our results suggest that different dynamin domains contribute to axonal transport and the sequestration of a pool of dynamin molecules in synaptic cytosol.
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