ArticleThe EMBO journal1998
The plasmid replicon of EBV consists of multiple cis-acting elements that facilitate DNA synthesis by the cell and a viral maintenance element.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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Who cites it
51 citing papers in PubMed, 118 citations in OpenAlex.
- Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cells.PLoS pathogens · 2013Trial
- Epidemiology and Inequality in the Incidence and Mortality of Nasopharynx Cancer in Asia.Osong public health and research perspectives · 2016Article
- Article
- Epstein-Barr virus latent genes.Experimental & molecular medicine · 2015Review
- Enhanced and prolonged baculovirus-mediated expression by incorporating recombinase system and in cis elements: a comparative study.Nucleic acids research · 2013Article
- Epstein-Barr nuclear antigen 1 (EBNA1)-dependent recruitment of origin recognition complex (Orc) on oriP of Epstein-Barr virus with purified proteins: stimulation by Cdc6 through its direct interaction with EBNA1.The Journal of biological chemistry · 2012Article
- Analysis of EBNA-1 and LMP-1 variants in diseases associated with EBV infection in Chinese children.Virology journal · 2012Article
- Article
- The extent of genetic diversity of Epstein-Barr virus and its geographic and disease patterns: a need for reappraisal.Virus research · 2009Review
- Zinc coordination is required for and regulates transcription activation by Epstein-Barr nuclear antigen 1.PLoS pathogens · 2009Article
- Identifying sites bound by Epstein-Barr virus nuclear antigen 1 (EBNA1) in the human genome: defining a position-weighted matrix to predict sites bound by EBNA1 in viral genomes.Journal of virology · 2009Article
- Epstein-Barr Nuclear Antigen 1 modulates replication of oriP-plasmids by impeding replication and transcription fork migration through the family of repeats.Virology journal · 2009Article
- Conditional gene vectors regulated in cis.Nucleic acids research · 2008Article
- An autonomous replicating element within the KSHV genome.Cell host & microbe · 2007Article
- Review
- The minimal replicator element of the Kaposi's sarcoma-associated herpesvirus terminal repeat supports replication in a semiconservative and cell-cycle-dependent manner.Journal of virology · 2007Article
- Latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus recruits uracil DNA glycosylase 2 at the terminal repeats and is important for latent persistence of the virus.Journal of virology · 2006Article
- Large-scale transfection of mammalian cells for the fast production of recombinant protein.Molecular biotechnology · 2006Review
- Identification of new human origins of DNA replication by an origin-trapping assay.Molecular and cellular biology · 2006Article
- Latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpesvirus interacts with origin recognition complexes at the LANA binding sequence within the terminal repeats.Journal of virology · 2006Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Plasmids containing oriP, the plasmid origin of Epstein-Barr virus (EBV), are replicated stably in human cells that express a single viral trans-acting factor, EBNA-1. Unlike plasmids of other viruses, but akin to human chromosomes, oriP plasmids are synthesized once per cell cycle, and are partitioned faithfully to daughter cells during mitosis. Although EBNA-1 binds multiple sites within oriP, its role in DNA synthesis and partitioning has been obscure. EBNA-1 lacks enzymatic activities that are present in the origin-binding proteins of other mammalian viruses, and does not interact with human cellular proteins that provide equivalent enzymatic functions. We demonstrate that plasmids with oriP or its constituent elements are synthesized efficiently in human cells in the absence of EBNA-1. Further, we show that human cells rapidly eliminate or destroy newly synthesized plasmids, and that both EBNA-1 and the family of repeats of oriP are required for oriP plasmids to escape this catastrophic loss. These findings indicate that EBV's plasmid replicon consists of genetic elements with distinct functions, multiple cis-acting elements that facilitate DNA synthesis and viral cis/trans elements that permit retention of replicated DNA in daughter cells. They also explain historical failures to identify mammalian origins of DNA synthesis as autonomously replicating sequences.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.