ArticleApplied and environmental microbiology1998
A small, dilute-cytoplasm, high-affinity, novel bacterium isolated by extinction culture and having kinetic constants compatible with growth at ambient concentrations of dissolved nutrients in seawater.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Challenges, adaptations, and biotechnological potential of oligophilic bacteria.Antonie van Leeuwenhoek · 2025Review
- Hydrocarbon metabolism and petroleum seepage as ecological and evolutionary drivers for Cycloclasticus.The ISME journal · 2025Article
- Risk assessment and bioburden evaluation of Agrobacterium tumefaciens-mediated transient protein expression in plants using the CaMV35S promoter.BMC biotechnology · 2023Article
- Bacterial transcriptional response to labile exometabolites from photosynthetic picoeukaryote Micromonas commoda.ISME communications · 2023Article
- A targeted liquid cultivation method for previously uncultured non-colony forming microbes.Frontiers in microbiology · 2023Article
- Impact of Microbial Uptake on the Nutrient Plume around Marine Organic Particles: High-Resolution Numerical Analysis.Microorganisms · 2022Article
- Microbial metabolites in the marine carbon cycle.Nature microbiology · 2022Review
- Ammonia-oxidizing archaea are integral to nitrogen cycling in a highly fertile agricultural soil.ISME communications · 2021Article
- High-throughput cultivation based on dilution-to-extinction with catalase supplementation and a case study of cultivating acI bacteria from Lake Soyang.Journal of microbiology (Seoul, Korea) · 2020Article
- Polycyclic Aromatic Hydrocarbon (PAH) Degradation Pathways of the Obligate Marine PAH Degrader Cycloclasticus sp. Strain P1.Applied and environmental microbiology · 2018Article
- Nano-Sized and Filterable Bacteria and Archaea: Biodiversity and Function.Frontiers in microbiology · 2018Review
- Engineered ribosomal RNA operon copy-number variants of E. coli reveal the evolutionary trade-offs shaping rRNA operon number.Nucleic acids research · 2015Article
- Kinetic parameters for nutrient enhanced crude oil biodegradation in intertidal marine sediments.Frontiers in microbiology · 2014Article
- RNA function and phosphorus use by photosynthetic organisms.Frontiers in plant science · 2013Review
- Dynamic autoinoculation and the microbial ecology of a deep water hydrocarbon irruption.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cooperation, competition, and coalitions in enzyme-producing microbes: social evolution and nutrient depolymerization rates.Frontiers in microbiology · 2012Article
- Flavobacterium johnsoniae as a model organism for characterizing biopolymer utilization in oligotrophic freshwater environments.Applied and environmental microbiology · 2011Article
- Genomic potential of Marinobacter aquaeolei, a biogeochemical "opportunitroph".Applied and environmental microbiology · 2011Article
- Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria.Nature · 2009Article
- An elusive marine photosynthetic bacterium is finally unveiled.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dilutions of raw seawater produced a bacterial isolate capable of extended growth in unamended seawater. Its 2.9-Mb genome size and 40-fg dry mass were similar to values for many naturally occurring aquatic organotrophs, but water and DNA comprised a large portion of this small chemoheterotroph, as compared to Escherichia coli. The isolate used only a few aromatic hydrocarbons and acetate, and glucose and amino acid incorporation were entirely absent, although many membrane and cytoplasmic proteins were inducible; it was named Cycloclasticus oligotrophus. A general rate equation that incorporates saturation phenomena into specific affinity theory is derived. It is used to relate the kinetic constants for substrate uptake by the isolate to its cellular proteins. The affinity constant KA for toluene was low at 1.3 microg/liter under optimal conditions, similar to those measured in seawater, and the low value was ascribed to an unknown slow step such as limitation by a cytoplasmic enzyme; KA increased with increasing specific affinities. Specific affinities, a degreess, were protocol sensitive, but under optimal conditions were 47.4 liters/mg of cells/h, the highest reported in the literature and a value sufficient for growth in seawater at concentrations sometimes found. Few rRNA operons, few cytoplasmic proteins, a small genome size, and a small cell size, coupled with a high a degreess and a low solids content and the ability to grow without intentionally added substrate, are consistent with the isolation of a marine bacterium with properties typical of the bulk of those present.
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