ArticleApplied and environmental microbiology1998
Monoclonal antibodies raised against native major capsid proteins of lactococcal c2-like bacteriophages.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- Characterization of theViruses · 2018Article
- Metagenomic Analysis of Dairy Bacteriophages: Extraction Method and Pilot Study on Whey Samples Derived from Using Undefined and Defined Mesophilic Starter Cultures.Applied and environmental microbiology · 2017Article
- Characterization of two polyvalent phages infecting Enterobacteriaceae.Scientific reports · 2017Article
- Characterization of Five Podoviridae Phages Infecting Citrobacter freundii.Frontiers in microbiology · 2016Article
- A novel approach for separating bacteriophages from other bacteriophages using affinity chromatography and phage display.Scientific reports · 2013Article
- Structure, adsorption to host, and infection mechanism of virulent lactococcal phage p2.Journal of virology · 2013Article
- Biology and genome sequence of Streptococcus mutans phage M102AD.Applied and environmental microbiology · 2012Article
- Purification and characterization of methyl parathion hydrolase from Burkholderia cepacia capable of degrading organophosphate insecticides.World journal of microbiology & biotechnology · 2012Article
- Purification of phage display-modified bacteriophage T4 by affinity chromatography.BMC biotechnology · 2011Article
- Evolution of Lactococcus lactis phages within a cheese factory.Applied and environmental microbiology · 2009Article
- Genome sequence and global gene expression of Q54, a new phage species linking the 936 and c2 phage species of Lactococcus lactis.Journal of bacteriology · 2006Article
- Biodiversity and classification of lactococcal phages.Applied and environmental microbiology · 2006Article
- Llama antibodies against a lactococcal protein located at the tip of the phage tail prevent phage infection.Journal of bacteriology · 2005Article
- Bacteriophage Tuc2009 encodes a tail-associated cell wall-degrading activity.Journal of bacteriology · 2004Article
- Multiplex PCR for detection and identification of lactococcal bacteriophages.Applied and environmental microbiology · 2000Article
- AbiQ, an abortive infection mechanism from Lactococcus lactis.Applied and environmental microbiology · 1998Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phage Q38, a representative member of the c2 species, was purified by CsCl gradient and used to immunize BALB/c mice. Monoclonal antibodies (MAbs) were raised and then characterized by enzyme-linked immunosorbent assay. Two MAbs of isotype immunoglobulin G2a, designated 2A5 and 6G7, reacted only with phages belonging to the c2 species and not with phages of the 936 and P335 species, with a Lactococcus lactis cell extract, or with phage DNA. Immunoelectron microscopy showed that both MAbs recognized only phage head proteins. They did not react with any denatured phage proteins in Western blot assays. However, when the nitrocellulose membranes were treated with a Triton-based buffer to assist in protein renaturation, MAbs 2A5 and 6G7 recognized the two major capsid proteins with molecular masses of 80 and 170 kDa. Competitive inhibition tests showed that the two MAbs bind to overlapping epitopes. These MAbs may be a useful tool for monitoring c2 bacteriophages during dairy fermentation and in genetic studies.
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