ArticleApplied and environmental microbiology1998
PCR detection of Coxiella burnetii from different clinical specimens, especially bovine milk, on the basis of DNA preparation with a silica matrix.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.
- Global and regional seroprevalence of coxiellosis in small ruminants: A systematic review and meta-analysis.Veterinary medicine and science · 2024Pooled it
- Molecular characterization ofJournal of advanced veterinary and animal research · 2023Article
- Molecular Detection ofTropical medicine and infectious disease · 2020Article
- Development of a Rapid and Sensitive Colorimetric Loop-Mediated Isothermal Amplification Assay: A Novel Technology for the Detection ofFrontiers in cellular and infection microbiology · 2020Article
- Article
- Bovine abortion caused byJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Biological characterization of Pasteurella multocida present in the Saiga population.BMC microbiology · 2019Article
- Molecular detection of Coxiella burnetii from the formalin-fixed tissues of Q fever patients with acute hepatitis.PloS one · 2017Article
- Anaplasma marginale and Theileria annulata in questing ticks from Portugal.Experimental & applied acarology · 2016Article
- Scientific opinion on an alternative method for the hygienic treatment of bovine colostrum through a series of filtration steps.EFSA journal. European Food Safety Authority · 2015Article
- Coxiella burnetii - Pathogenic Agent of Q (Query) Fever.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2014Article
- Q fever abortions in ruminants and associated on-farm risk factors in northern Cyprus.BMC veterinary research · 2011Article
- Comparison of PCR, immunofluorescence assay, and pathogen isolation for diagnosis of q fever in humans with spontaneous abortions.Journal of clinical microbiology · 2008Article
- High throughput detection of Coxiella burnetii by real-time PCR with internal control system and automated DNA preparation.BMC microbiology · 2008Article
- Prevalence of Coxiella burnetii DNA in vaginal and uterine samples from healthy cats of north-central Colorado.Journal of feline medicine and surgery · 2007Article
- Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii.BMC microbiology · 2006Article
- Detection of Coxiella burnetii by PCR in mice after administration of live M-44 vaccine.Folia microbiologica · 2003Article
- Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR.Journal of clinical microbiology · 2001Article
- Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa.Journal of clinical microbiology · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For PCR detection of Coxiella burnetii in various clinical specimens we developed a sample preparation method in which silica binding of DNA was used. This method was found to be fast, easily performed with large numbers of samples, and equally sensitive for all of the specimens tested (livers, spleens, placentas, heart valves, milk, blood). The DNA preparation method described here can also be used as an initial step in any PCR-based examination of specimens. The procedure was tested with more than 600 milk samples, which were taken from 21 cows that were seropositive for C. burnetii and reportedly had fertility problems (and therefore were suspected of shedding the agent through milk intermittently or continuously). Of the 21 cows tested, 6 were shedding C. burnetii through milk. Altogether, C. burnetii DNA was detected in 6% of the samples. There was no correlation between the shedding pattern and the serological results.
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