ArticleAntimicrobial agents and chemotherapy1998
Effect of disruption of a gene encoding an autolysin of Enterococcus faecalis OG1RF.
Article in Antimicrobial agents and chemotherapy, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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Who cites it
37 citing papers in PubMed, 56 citations in OpenAlex.
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- Insights into ecology, pathogenesis, and biofilm formation ofMicrobiology and molecular biology reviews : MMBR · 2025Review
- A moonlighting role for LysM peptidoglycan binding domains underpins Enterococcus faecalis daughter cell separation.Communications biology · 2023Article
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- Biochemical Characterizations of the Putative Endolysin Ecd09610 Catalytic Domain fromAntibiotics (Basel, Switzerland) · 2022Article
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- Genome-Wide Mutagenesis Identifies Factors Involved in Enterococcus faecalis Vaginal Adherence and Persistence.Infection and immunity · 2020Article
- Intestinal Bile Acids Induce a Morphotype Switch in Vancomycin-Resistant Enterococcus that Facilitates Intestinal Colonization.Cell host & microbe · 2019Article
- Biofilm-associated infection by enterococci.Nature reviews. Microbiology · 2019Review
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- Eep confers lysozyme resistance to enterococcus faecalis via the activation of the extracytoplasmic function sigma factor SigV.Journal of bacteriology · 2013Article
- AhrC and Eep are biofilm infection-associated virulence factors in Enterococcus faecalis.Infection and immunity · 2013Article
- Article
- Deletion of the glycosyltransferase bgsB of Enterococcus faecalis leads to a complete loss of glycolipids from the cell membrane and to impaired biofilm formation.BMC microbiology · 2011Article
- Enterococcal biofilm formation and virulence in an optimized murine model of foreign body-associated urinary tract infections.Infection and immunity · 2010Article
- Characterization of Acp, a peptidoglycan hydrolase of Clostridium perfringens with N-acetylglucosaminidase activity that is implicated in cell separation and stress-induced autolysis.Journal of bacteriology · 2010Article
- Contribution of autolysin and Sortase a during Enterococcus faecalis DNA-dependent biofilm development.Infection and immunity · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
A mutant (TX5127) of Enterococcus faecalis OG1RF was generated by disruption mutagenesis of a previously described autolysin gene. TX5127 formed longer chains (2 to 10 cells per chain) than wild-type OG1RF (mainly single cells) during growth in broth even though it had a growth rate similar to that of the parental strain as measured by turbidity and cell count. Autolysin activity, as defined by the ability to lyse heat-killed Micrococcus lysodeikticus cells, was absent in TX5127, while this activity was easily detectable in OG1RF. However, disruption of this autolysin gene did not block the ability of TX5127 to hydrolyze E. faecalis cell walls compared to that of OG1RF. The autolysis rate of cells of TX5127 in 10 mM sodium phosphate buffer (pH 6.8) was slower than that of wild-type OG1RF. TX5127 also showed a decreased rate of lysis in the presence of penicillin, as measured by changes in the turbidity of the culture during 24 h of incubation at 37 degrees C and a slightly decreased effect of penicillin as measured by time-kill curves. The virulence of TX5127 was similar to that of OG1RF in the mouse peritonitis model, indicating that the autolysin of E. faecalis is not important for infection in this model.
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