ArticleThe Biochemical journal1998
Glycosylphosphatidylinositol-dependent secretory transport in Trypanosoma brucei.
Article in The Biochemical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 90 citations in OpenAlex.
- Mechanisms of Quality Control in GPI-Anchored Proteins: The Central Role of the Lipid Anchor.International journal of molecular sciences · 2026Review
- Investigation of Intercellular Trafficking of Global Glycosylphosphatidylinositol-Anchored Proteins Using Cell Metabolic Engineering and Transwell Coculture.Angewandte Chemie (International ed. in English) · 2026Article
- Multifunctional roles of Sec13 paralogues in the euglenozoanOpen biology · 2025Article
- A high-throughput protein tagging toolkit that retains endogenous untranslated regions for studying gene regulation in kinetoplastids.Open biology · 2025Article
- Multifunctional Roles of Sec13 Paralogues in the EuglenozoanbioRxiv : the preprint server for biology · 2024Article
- Spin-labeling Insights into How Chemical Fixation Impacts Glycan Organization on Cells.Applied magnetic resonance · 2024Article
- Article
- Fatty acid uptake inFrontiers in cellular and infection microbiology · 2022Review
- Extravascular Dermal Trypanosomes in Suspected and Confirmed Cases of gambiense Human African Trypanosomiasis.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Observational
- Cyclophilin 19 secreted in the host cell cytosol by Trypanosoma cruzi promotes ROS production required for parasite growth.Cellular microbiology · 2021Article
- Late ESCRT machinery mediates the recycling and Rescue of Invariant Surface Glycoprotein 65 in Trypanosoma brucei.Cellular microbiology · 2020Article
- Rab11 mediates selective recycling and endocytic trafficking in Trypanosoma brucei.Traffic (Copenhagen, Denmark) · 2018Article
- Identification of di-substituted ureas that prevent growth of trypanosomes through inhibition of translation initiation.Scientific reports · 2018Article
- Quantitative Proteomic Analysis of Replicative and Nonreplicative Forms Reveals Important Insights into Chromatin Biology of Trypanosoma cruzi.Molecular & cellular proteomics : MCP · 2017Article
- Evaluation of Antigens for Development of a Serological Test for Human African Trypanosomiasis.PloS one · 2016Article
- A membrane-bound eIF2 alpha kinase located in endosomes is regulated by heme and controls differentiation and ROS levels in Trypanosoma cruzi.PLoS pathogens · 2015Article
- Acylation in trypanosomatids: an essential process and potential drug target.Trends in parasitology · 2014Review
- Mouse infection and pathogenesis by Trypanosoma brucei motility mutants.Cellular microbiology · 2014Article
- Characterization of the late endosomal ESCRT machinery in Trypanosoma brucei.Traffic (Copenhagen, Denmark) · 2013Article
- Inhibition of nucleotide sugar transport in Trypanosoma brucei alters surface glycosylation.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have investigated the role of glycosylphosphatidylinositol (GPI) anchors in forward secretory trafficking using African trypanosomes as a model system. Soluble GPI-minus forms of variant surface glycoprotein (VSG), in which the C-terminal GPI-addition peptide signal is deleted, are secreted from transformed procyclic trypanosomes with 5-fold reduced kinetics, relative to matched GPI-anchored constructs. Cell fractionation and immunofluorescence localization studies indicate that the GPI-minus VSG reporters accumulate in the endoplasmic reticulum (ER). This transport defect is specific, since overexpression of GPI-minus VSG has no effect on the rate of transport of a second soluble secretory reporter (BiPN) when co-expressed in the same cells. Two results suggest that delayed forward transport cannot be accounted for by failure to fold/assemble in the absence of a GPI anchor, thereby leading to prolonged association with ER quality-control machinery. First, no evidence was found for elevated association of GPI-minus VSG with the ER molecular chaperone, BiP. Secondly, newly synthesized GPI-minus VSG is dimerized efficiently, as judged by velocity-sedimentation analysis. GPI-dependent transport is not confined to the VSG reporters, because a similar dependence is found with another trypanosomal GPI-anchored protein, trans-sialidase. These findings suggest that GPI structures act in a positive manner to mediate efficient forward transport of some, and perhaps all, GPI-anchored proteins in the early secretory pathway of trypanosomes. Possible mechanisms for GPI-dependent transport are discussed with respect to current models of vesicular trafficking.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.