ArticleProceedings of the National Academy of Sciences of the United States of America1998
Cloning of the cDNA encoding the large subunit of human RNase HI, a homologue of the prokaryotic RNase HII.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 67 citations in OpenAlex.
- Gapmers: Invention and Early History.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Ribonuclease H2 Subunit A Preserves Genomic Integrity and Promotes Prostate Cancer Progression.Cancer research communications · 2022Article
- Review
- Human RNase H1 is associated with protein P32 and is involved in mitochondrial pre-rRNA processing.PloS one · 2013Article
- The hepatitis B virus ribonuclease H is sensitive to inhibitors of the human immunodeficiency virus ribonuclease H and integrase enzymes.PLoS pathogens · 2013Article
- Article
- Functional consequences of the RNase H2A subunit mutations that cause Aicardi-Goutieres syndrome.The Journal of biological chemistry · 2011Article
- PCNA directs type 2 RNase H activity on DNA replication and repair substrates.Nucleic acids research · 2011Article
- The structural and biochemical characterization of human RNase H2 complex reveals the molecular basis for substrate recognition and Aicardi-Goutières syndrome defects.The Journal of biological chemistry · 2011Article
- Crystal structures of RNase H2 in complex with nucleic acid reveal the mechanism of RNA-DNA junction recognition and cleavage.Molecular cell · 2010Article
- The structure of the mammalian RNase H2 complex provides insight into RNA.NA hybrid processing to prevent immune dysfunction.The Journal of biological chemistry · 2010Article
- Aicardi-Goutieres syndrome and related phenotypes: linking nucleic acid metabolism with autoimmunity.Human molecular genetics · 2009Review
- Ribonuclease H: the enzymes in eukaryotes.The FEBS journal · 2009Review
- New roles for the major human 3'-5' exonuclease TREX1 in human disease.Cell cycle (Georgetown, Tex.) · 2008Review
- RNase H2 of Saccharomyces cerevisiae is a complex of three proteins.Nucleic acids research · 2004Article
- Selective inhibitory DNA aptamers of the human RNase H1.Nucleic acids research · 2003Article
- Excision of misincorporated ribonucleotides in DNA by RNase H (type 2) and FEN-1 in cell-free extracts.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Increased efficacy of antileishmanial antisense phosphorothioate oligonucleotides in Leishmania amazonensis overexpressing ribonuclease H.Biochemical pharmacology · 2001Article
- Review
- Eukaryotic ribonucleases HI and HII generate characteristic hydrolytic patterns on DNA-RNA hybrids: further evidence that mitochondrial RNase H is an RNase HII.Nucleic acids research · 2000Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two RNases H of mammalian tissues have been described: RNase HI, the activity of which was found to rise during DNA replication, and RNase HII, which may be involved in transcription. RNase HI is the major mammalian enzyme representing around 85% of the total RNase H activity in the cell. By using highly purified calf thymus RNase HI we identified the sequences of several tryptic peptides. This information enabled us to determine the sequence of the cDNA coding for the large subunit of human RNase HI. The corresponding ORF of 897 nt defines a polypeptide of relative molecular mass of 33,367, which is in agreement with the molecular mass obtained earlier by SDS/PAGE. Expression of the cloned ORF in Escherichia coli leads to a polypeptide, which is specifically recognized by an antiserum raised against calf thymus RNase HI. Interestingly, the deduced amino acid sequence of this subunit of human RNase HI displays significant homology to RNase HII from E. coli, an enzyme of unknown function and previously judged as a minor activity. This finding suggests an evolutionary link between the mammalian RNases HI and the prokaryotic RNases HII. The idea of a mammalian RNase HI large subunit being a strongly conserved protein is substantiated by the existence of homologous ORFs in the genomes of other eukaryotes and of all eubacteria and archaebacteria that have been completely sequenced.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.