ArticleBiophysical journal1998
Membrane capacitance changes associated with particle uptake during phagocytosis in macrophages.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 100 citations in OpenAlex.
- Phagocytosis at a glance.Journal of cell science · 2025Review
- Article
- Biomechanics of phagocytosis of red blood cells by macrophages in the human spleen.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Membrane backtracking at the maximum capacity of nondigestible antigen phagocytosis in macrophages.Biophysical journal · 2023Article
- PKC-ε regulates vesicle delivery and focal exocytosis for efficient IgG-mediated phagocytosis.Journal of cell science · 2021Article
- Origins of eukaryotic excitability.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Examining the Underappreciated Role ofFrontiers in immunology · 2021Review
- A Multiparametric and High-Throughput Assay to Quantify the Influence of Target Size on Phagocytosis.Biophysical journal · 2019Article
- Membrane dynamics and organelle biogenesis-lipid pipelines and vesicular carriers.BMC biology · 2017Article
- Maxed out macs: physiologic cell clearance as a function of macrophage phagocytic capacity.The FEBS journal · 2017Review
- CaThe Journal of biological chemistry · 2017Article
- Comparative Anatomy of Phagocytic and Immunological Synapses.Frontiers in immunology · 2016Review
- Lysosomal exocytosis and lipid storage disorders.Journal of lipid research · 2014Review
- The cell surface proteome of Entamoeba histolytica.Molecular & cellular proteomics : MCP · 2014Article
- A TRP channel in the lysosome regulates large particle phagocytosis via focal exocytosis.Developmental cell · 2013Article
- Plasma membrane tension orchestrates membrane trafficking, cytoskeletal remodeling, and biochemical signaling during phagocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Cdc42 interacts with the exocyst complex to promote phagocytosis.The Journal of cell biology · 2013Article
- Quantitative proteomics reveals that only a subset of the endoplasmic reticulum contributes to the phagosome.Molecular & cellular proteomics : MCP · 2012Article
- A novel function for SNAP29 (synaptosomal-associated protein of 29 kDa) in mast cell phagocytosis.PloS one · 2012Article
- The zipper mechanism in phagocytosis: energetic requirements and variability in phagocytic cup shape.BMC systems biology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We report the use of capacitance measurements to monitor particle uptake after cellular exposure to phagocytic stimuli. In these studies, human monocyte-derived macrophages (HMDMs) and cells from the murine macrophage-like cell line J774.1 were exposed to immune complexes or sized latex particles (0.8 or 3.2 micron in diameter). An average decrease in cell capacitance of 8 pF was seen after exposure of the cells to immune complexes. Cells in which particle uptake was inhibited by cytochalasin B treatment before exposure to immune complexes showed an average increase of 0.5 pF. The decrease in membrane capacitance after exposure of cells to particulate stimuli was absent with the soluble stimulus, platelet-activating factor, further confirming that decreases in membrane capacitance were due to particle uptake. Exposure of cells to sized latex particles resulted in a graded, stepwise decrease in membrane capacitance. The average step size for 0.8-micron particles was 250 fF, and the average step change for the larger 3.2-micron particles was 480 fF, as calculated from Gaussian fits to the step size amplitude histograms. The predicted step size for the individual particles based upon the minimum amount of membrane required to enclose a particle and a specific capacitance of 10 fF/micron2 was 20 and 320 fF, respectively. The step size for the smaller particles deviates significantly from the predicted size distribution, indicating either a possible lower limit to the size of the phagocytic vacuole or multiple particles taken up within a single phagosome. Dynamic interaction between phagocytosis and exocytosis was observed in a number of cells as a biphasic response consisting of an initial rapid increase in capacitance, consistent with cellular exocytosis, followed by stepwise decreases in capacitance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.