ArticleBiophysical journal1998
Fluorescence correlation microscopy of cells in the presence of autofluorescence.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Study on intracellular delivery of liposome encapsulated quantum dots using advanced fluorescence microscopy.Scientific reports · 2019Article
- There and back again: from the origin of life to single molecules.European biophysics journal : EBJ · 2018Article
- Bayesian model selection applied to the analysis of fluorescence correlation spectroscopy data of fluorescent proteins in vitro and in vivo.Analytical chemistry · 2015Article
- Discrepancy between fluorescence correlation spectroscopy and fluorescence recovery after photobleaching diffusion measurements of G-protein-coupled receptors.Analytical biochemistry · 2013Article
- Quantitative imaging of cerebral blood flow velocity and intracellular motility using dynamic light scattering-optical coherence tomography.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2013Article
- Quantitative analysis of self-association and mobility of annexin A4 at the plasma membrane.Biophysical journal · 2013Article
- Analyzing the homeostasis of signaling proteins by a combination of Western blot and fluorescence correlation spectroscopy.Biophysical journal · 2011Article
- Coclustering of ErbB1 and ErbB2 revealed by FRET-sensitized acceptor bleaching.Biophysical journal · 2010Article
- Fluorescence fluctuation spectroscopy: ushering in a new age of enlightenment for cellular dynamics.Biophysical reviews · 2009Article
- Biliverdin reductase is a transporter of haem into the nucleus and is essential for regulation of HO-1 gene expression by haematin.The Biochemical journal · 2008Article
- Intranuclear binding kinetics and mobility of single native U1 snRNP particles in living cells.Molecular biology of the cell · 2006Article
- Microenvironment and effect of energy depletion in the nucleus analyzed by mobility of multiple oligomeric EGFPs.Biophysical journal · 2006Article
- In vivo quantitative studies of dynamic intracellular processes using fluorescence correlation spectroscopy.Biophysical journal · 2006Article
- Rotational and translational diffusion of peptide-coated CdSe/CdS/ZnS nanorods studied by fluorescence correlation spectroscopy.Journal of the American Chemical Society · 2006Article
- Sampling effects, noise, and photobleaching in temporal image correlation spectroscopy.Biophysical journal · 2006Article
- Integrating nuclear receptor mobility in models of gene regulation.Nuclear receptor signaling · 2006Article
- CD8 kinetically promotes ligand binding to the T-cell antigen receptor.Biophysical journal · 2005Article
- Cross talk free fluorescence cross correlation spectroscopy in live cells.Biophysical journal · 2005Article
- Unraveling protein-protein interactions in living cells with fluorescence fluctuation brightness analysis.Biophysical journal · 2005Article
- Global analysis of fluorescence fluctuation data.European biophysics journal : EBJ · 2005Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence correlation microscopy (FCM), the combination of fluorescence correlation spectroscopy (FCS) and digital microscopy (Brock and Jovin, 1998. Cell. Mol. Biol. 44:847-856), has been implemented for measuring molecular diffusion and association in living cells with explicit consideration of autocorrelations arising from autofluorescence. Autofluorescence excited at 532 nm colocalizes with mitochondria, has flavin-like spectral characteristics, exhibits relaxation times characteristic for the diffusion of high-molecular-weight proteins, and depends on the incubation conditions of the cells. These time- and location-dependent properties preclude the assignment of universal background parameters. The lower limit for detection of microinjected dextran molecules labeled with the carboxymethylindocyanine dye Cy3 was a few thousand molecules per cell, and the diffusion constant of 1.7 x 10(-7) cm2/s agreed well with values measured with other methods. Based on the fluorescence signal per molecule (fpm) and the molecule number derived from autocorrelation analysis, a new method is devised to define intracellular association states. We conclude that FCM is a powerful, noninvasive method for probing molecular interactions in femtoliter volume elements within defined subcellular locations in living cells.
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