ArticleMolecular and cellular biochemistry1998
Retinol uptake and metabolism, and cellular retinol binding protein expression in an in vitro model of hepatic stellate cells.
Article in Molecular and cellular biochemistry, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 34 citations in OpenAlex.
- Genetic and Molecular Characterization of the Immortalized Murine Hepatic Stellate Cell Line GRX.Cells · 2022Article
- Silencing of tissue factor by antisense deoxyoligonucleotide mitigates thioacetamide-induced liver injury.Naunyn-Schmiedeberg's archives of pharmacology · 2020Article
- Probiotics promote rapid-turnover protein production by restoring gut flora in patients with alcoholic liver cirrhosis.Hepatology international · 2013Article
- Article
- Antagonism of the chemokine Ccl5 ameliorates experimental liver fibrosis in mice.The Journal of clinical investigation · 2010Article
- Changes in E-NTPDase 3 expression and extracellular nucleotide hydrolysis during the myofibroblast/lipocyte differentiation.Molecular and cellular biochemistry · 2010Article
- Lycopene isomerisation and storage in an in vitro model of murine hepatic stellate cells.European journal of nutrition · 2009Article
- Activity and expression of ecto-nucleotide pyrophosphate/phosphodiesterases in a hepatic stellate cell line.Molecular and cellular biochemistry · 2009Article
- Resveratrol inhibits cell growth by inducing cell cycle arrest in activated hepatic stellate cells.Molecular and cellular biochemistry · 2008Article
- Variations of ganglioside biosynthetic pathways in the phenotype conversion from myofibroblasts to lipocytes in murine hepatic stellate cell line.Molecular and cellular biochemistry · 2007Article
- Effect of pentoxifylline on arachidonic acid metabolism, neutral lipid synthesis and accumulation during induction of the lipocyte phenotype by retinol in murine hepatic stellate cell.Molecular and cellular biochemistry · 2003Article
- Effects of retinoic acid on proliferation, phenotype and expression of cyclin-dependent kinase inhibitors in TGF-beta1-stimulated rat hepatic stellate cells.World journal of gastroenterology · 2000Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liver is a major site of retinoid metabolism and storage, and more than 80% of the liver retinoids are stored in hepatic stellate cells. These cells represent less than 1% of the total liver protein, reaching a very high relative intracellular retinoid concentration. The plasma level of retinol is maintained close to 2 microM, and hepatic stellate cells have to be able both to uptake or to release retinol depending upon the extracellular retinol status. In view of their paucity in the liver tissue, stellate cells have been studied in primary cultures, in which they loose rapidly the stored lipids and retinol, and convert spontaneously into the activated myofibroblast phenotype, turning a long-term study of their retinol metabolism impossible. We have analyzed the retinol metabolism in the established GRX cell line, representative of stellate cells. We showed that this cell line behaves very similarly, with respect the retinol uptake and release, to primary cultures of hepatic stellate cells. Moreover, we showed that the cellular retinol binding protein (CRBP-I) expression in these cells, relevant for both uptake and esterification of retinol, responds to the extracellular retinol status, and is correlated to the retinol binding capacity of the cytosol. Its expression is not associated with the overall induction of the lipocyte phenotype by other agents. We conclude that the GRX cell line represents an in vitro model of hepatic stellate cells, and responds very efficiently to wide variations of the extracellular retinol status by autonomous controls of its uptake, storage or release.
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