ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1998
Localization of myosin-Ibeta near both ends of tip links in frog saccular hair cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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Who cites it
40 citing papers in PubMed, 93 citations in OpenAlex.
- Chiral growth of adherent filopodia.Biophysical journal · 2023Article
- The role of motor proteins in photoreceptor protein transport and visual function.Ophthalmic genetics · 2022Article
- Electron Microscopy Techniques for Investigating Structure and Composition of Hair-Cell Stereociliary Bundles.Frontiers in cell and developmental biology · 2021Article
- Serial scanning electron microscopy of anti-PKHD1L1 immuno-gold labeled mouse hair cell stereocilia bundles.Scientific data · 2020Article
- A cryo-tomography-based volumetric model of the actin core of mouse vestibular hair cell stereocilia lacking plastin 1.Journal of structural biology · 2020Article
- Unconventional Myosins: How Regulation Meets Function.International journal of molecular sciences · 2019Review
- A Bundle of Mechanisms: Inner-Ear Hair-Cell Mechanotransduction.Trends in neurosciences · 2019Review
- The elusive mechanotransduction machinery of hair cells.Current opinion in neurobiology · 2015Review
- The molecules that mediate sensory transduction in the mammalian inner ear.Current opinion in neurobiology · 2015Review
- The physiology of mechanoelectrical transduction channels in hearing.Physiological reviews · 2014Review
- Regulation of the transient receptor potential channel TRPA1 by its N-terminal ankyrin repeat domain.Journal of molecular modeling · 2013Article
- Sensing sound: molecules that orchestrate mechanotransduction by hair cells.Trends in neurosciences · 2012Review
- Integrating the biophysical and molecular mechanisms of auditory hair cell mechanotransduction.Nature communications · 2011Review
- Myosin VIIa and sans localization at stereocilia upper tip-link density implicates these Usher syndrome proteins in mechanotransduction.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Myo1c mutations associated with hearing loss cause defects in the interaction with nucleotide and actin.Cellular and molecular life sciences : CMLS · 2011Article
- Principles of unconventional myosin function and targeting.Annual review of cell and developmental biology · 2011Review
- The R109H variant of fascin-2, a developmentally regulated actin crosslinker in hair-cell stereocilia, underlies early-onset hearing loss of DBA/2J mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Review series: The cell biology of hearing.The Journal of cell biology · 2010Review
- Myosin motor function: the ins and outs of actin-based membrane protrusions.Cellular and molecular life sciences : CMLS · 2010Review
- Localisation of the mechanotransducer channels in mammalian cochlear hair cells provides clues to their gating.The Journal of physiology · 2010Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Current evidence suggests that the adaptation motor of mechanoelectrical transduction in vertebrate hair cells is myosin-Ibeta. Previously, confocal and electron microscopy of bullfrog saccular hair cells using an anti-myosin-Ibeta antibody labeled the tips of stereocilia. We have now done quantitative immunoelectron microscopy to test whether myosin-Ibeta is enriched at or near the side plaques of tip links, the proposed sites of adaptation, using hair bundles that were serially sectioned parallel to the macular surface. The highest particle density occurred at stereocilia bases, close to the cuticular plate. Also, stereocilia of differing lengths had approximately the same number of total particles, suggesting equal targeting of myosin-Ibeta to all stereocilia. Finally, particles tended to clump in clusters of two to five particles in the distal two-thirds of stereocilia, suggesting a tendency for self-assembly of myosin-Ibeta. As expected from fluorescence microscopy, particle density was high in the distal 1 micrometer of stereocilia. If myosin-Ibeta is the adaptation motor, a difference should exist in particle density between regions containing the side plaque and those excluding it. Averaging of particle distributions revealed two regions with approximately twice the average density: at the upper ends of tip links in a 700-nm-long region centered approximately 100 nm above the side plaque, and at the lower ends of tip links within the tip plaques. Controls demonstrated no such increase. The shortest stereocilia, which lack side plaques, showed no concentration rise on their sides. Thus, the specific localization of myosin-Ibeta at both ends of tip links supports its role as the adaptation motor.
Indexed as
Identifiers
9786971PMC6793541W1893639807What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.