ArticleThe Journal of cell biology1998
Identification of Kel1p, a kelch domain-containing protein involved in cell fusion and morphology in Saccharomyces cerevisiae.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 94 citations in OpenAlex.
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- Bidirectional Genetic Control of Phenotypic Heterogeneity and Its Implication for Cancer Drug Resistance.Molecular biology and evolution · 2021Article
- The Smk1 MAPK and Its Activator, Ssp2, Are Required for Late Prospore Membrane Development in SporulatingJournal of fungi (Basel, Switzerland) · 2021Article
- PoRal2 Is Involved in Appressorium Formation and VirulenceFrontiers in plant science · 2021Article
- A Noncanonical Hippo Pathway Regulates Spindle Disassembly and Cytokinesis During Meiosis inGenetics · 2020Article
- A Predicted Mannoprotein Participates inmSphere · 2018Article
- Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors inFrontiers in microbiology · 2018Article
- Membrane curvature directs the localization of Cdc42p to novel foci required for cell-cell fusion.The Journal of cell biology · 2017Article
- Analysis of transcriptional profiles of Saccharomyces cerevisiae exposed to bisphenol A.Current genetics · 2017Article
- A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.PLoS genetics · 2017Article
- The final cut: cell polarity meets cytokinesis at the bud neck in S. cerevisiae.Cellular and molecular life sciences : CMLS · 2016Review
- Timely Closure of the Prospore Membrane Requires SPS1 and SPO77 in Saccharomyces cerevisiae.Genetics · 2016Article
- Article
- Cell biology of yeast zygotes, from genesis to budding.Biochimica et biophysica acta · 2015Review
- Plasmids for C-terminal tagging in Saccharomyces cerevisiae that contain improved GFP proteins, Envy and Ivy.Yeast (Chichester, England) · 2015Article
- A protein complex containing Epo1p anchors the cortical endoplasmic reticulum to the yeast bud tip.The Journal of cell biology · 2015Article
- Article
- SPO73 and SPO71 Function Cooperatively in Prospore Membrane Elongation During Sporulation in Saccharomyces cerevisiae.PloS one · 2015Article
- Saccharomyces cerevisiae Kelch proteins and Bud14 protein form a stable 520-kDa formin regulatory complex that controls actin cable assembly and cell morphogenesis.The Journal of biological chemistry · 2014Article
- Targeted disruption of an EH-domain protein endocytic complex, Pan1-End3.Traffic (Copenhagen, Denmark) · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We showed previously that protein kinase C, which is required to maintain cell integrity, negatively regulates cell fusion (Philips, J., and I. Herskowitz. 1997. J. Cell Biol. 138:961-974). To identify additional genes involved in cell fusion, we looked for genes whose overexpression relieved the defect caused by activated alleles of Pkc1p. This strategy led to the identification of a novel gene, KEL1, which encodes a protein composed of two domains, one containing six kelch repeats, a motif initially described in the Drosophila protein Kelch (Xue, F., and L. Cooley. 1993. Cell. 72:681- 693), and another domain predicted to form coiled coils. Overexpression of KEL1 also suppressed the defect in cell fusion of spa2Delta and fps1Delta mutants. KEL2, which corresponds to ORF YGR238c, encodes a protein highly similar to Kel1p. Its overexpression also suppressed the mating defect associated with activated Pkc1p. Mutants lacking KEL1 exhibited a moderate defect in cell fusion that was exacerbated by activated alleles of Pkc1p or loss of FUS1, FUS2, or FPS1, but not by loss of SPA2. kel1Delta mutants form cells that are elongated and heterogeneous in shape, indicating that Kel1p is also required for proper morphology during vegetative growth. In contrast, kel2Delta mutants were not impaired in cell fusion or morphology. Both Kel1p and Kel2p localized to the site where cell fusion occurs during mating and to regions of polarized growth during vegetative growth. Coimmunoprecipitation and two-hybrid analyses indicated that Kel1p and Kel2p physically interact. We conclude that Kel1p has a role in cell morphogenesis and cell fusion and may antagonize the Pkc1p pathway.
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