ArticleInfection and immunity1998
1,25-Dihydroxyvitamin D3 induces nitric oxide synthase and suppresses growth of Mycobacterium tuberculosis in a human macrophage-like cell line.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03984890 (Effect of Vitamin D3 Supplementation on Chronic Granulomatous Disease Patients With BCGosis/Itis), which is not on this map. Cited by 103 papers, 4 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effect of Vitamin D3 Supplementation on Chronic Granulomatous Disease Patients With BCGosis/Itis
Who cites it
103 citing papers in PubMed, 4 syntheses or guidelines pooled it, 304 citations in OpenAlex.
- Pooled it
- Vitamin DBMC infectious diseases · 2023Pooled it
- Vitamin D deficiency and clinical outcomes related to septic shock in children with critical illness: a systematic review.European journal of clinical nutrition · 2019Pooled it
- Pooled it
- Vitamin D, tuberculin skin test conversion, and latent tuberculosis in Mongolian school-age children: a randomized, double-blind, placebo-controlled feasibility trial.The American journal of clinical nutrition · 2012Trial
- High-dose vitamin D(3) during intensive-phase antimicrobial treatment of pulmonary tuberculosis: a double-blind randomised controlled trial.Lancet (London, England) · 2011Trial
- Serum vitamin D and calcium levels among tuberculosis patients in Southern Ethiopia: a comparative cross-sectional study.BMJ open · 2025Article
- Immunomodulatory Effects of Vitamin D and Zinc on Viral Infection.Biological trace element research · 2025Review
- Ex Vivo Immune Function and Modulatory Effects of Calcitriol in Dogs with Naturally Occurring Diabetes Mellitus.Veterinary sciences · 2024Article
- Comparative Study of Vitamin D Levels in Newly Diagnosed Tuberculosis and a Normal Population.Medicina (Kaunas, Lithuania) · 2024Article
- An Updated Trial Sequential Meta-analysis of Vitamin D Receptor Gene Polymorphism (Fok1, Bsm1, Taq1 and Apa1) and Risk to Tuberculosis.Indian journal of clinical biochemistry : IJCB · 2024Article
- Evaluation of serum 25-hydroxyvitamin D, C-reactive protein, and haptoglobin as biomarkers in dogs newly diagnosed with histoplasmosis.Journal of veterinary internal medicine · 2023Article
- Serum 25-hydroxyvitamin D concentrations in dogs with coccidioidomycosis and variables associated with extent of clinically evident disease.Journal of veterinary internal medicine · 2023Article
- Research progress on genetic control of host susceptibility to tuberculosis.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2022Review
- Impact of Vitamin D in Prophylaxis and Treatment in Tuberculosis Patients.International journal of molecular sciences · 2022Review
- High Vitamin D Concentrations Restore the Ability to Express LL37 byBiomolecules · 2022Article
- Monthly Increase in Vitamin D Levels upon Supplementation with 2000 IU/Day in Healthy Volunteers: Result from "Integriamoci", a Pilot Pharmacokinetic Study.Molecules (Basel, Switzerland) · 2022Article
- Impaired spatial memory in adult vitamin D deficient BALB/c mice is associated with reductions in spine density, nitric oxide, and neural nitric oxide synthase in the hippocampus.AIMS neuroscience · 2022Article
- Association of Vitamin D Deficiency With Pulmonary Tuberculosis: A Systematic Review and Meta-Analysis.Cureus · 2021Review
- Molecular mechanism of interaction ofBiochemistry and biophysics reports · 2021Article
43 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inducible synthesis of nitric oxide (NO) by macrophages is an important mechanism of the host defense against intracellular infection in mice, but the evidence for significant levels of inducible NO production by human macrophages is controversial. Here we report that the human promyelocytic cell line HL-60, when differentiated to a macrophage-like phenotype, acquires the ability to produce substantial amounts of NO on stimulation with LPS or 1, 25-dihydroxyvitamin D3 (1,25-D3) in the absence of activating factors such as gamma interferon. Expression of the inducible nitric oxide synthase (NOS2) was confirmed by sequencing of the reverse transcription-PCR product from stimulated HL-60 cells. Kinetic studies after lipopolysaccharide stimulation show that NOS2 mRNA levels rise within 3 to 6 h, that conversion of [14C]arginine to [14C]citrulline is maximal at 5 to 6 days, and that levels of reactive nitrogen intermediates stabilize at around 20 microM at 7 to 8 days. We find that 1,25-D3 acts to suppress the growth of Mycobacterium tuberculosis in these cells and that this effect is inhibited by NG-monomethyl-L-arginine, suggesting that vitamin D-induced NO production may play a role in the host defense against human tuberculosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.