ArticleInfection and immunity1998
Altered expression of selectable marker URA3 in gene-disrupted Candida albicans strains complicates interpretation of virulence studies.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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90 citing papers in PubMed.
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- Cdc14 phosphatase contributes to cell wall integrity and pathogenesis inFrontiers in microbiology · 2023Article
- Targeted Genetic Changes in Candida albicans Using Transient CRISPR-Cas9 Expression.Current protocols · 2021Article
- CRISPR-Based Genetic Manipulation ofFrontiers in genome editing · 2020Review
- Titrating Gene Function in the Human Fungal Pathogen Candida albicans through Poly-Adenosine Tract Insertion.mSphere · 2019Article
- Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi.Fungal biology and biotechnology · 2019Article
- Candida albicans white and opaque cells exhibit distinct spectra of organ colonization in mouse models of infection.PloS one · 2019Article
- Candida albicans Cannot Acquire Sufficient Ethanolamine from the Host To Support Virulence in the Absence ofInfection and immunity · 2018Article
- Loss of Upc2p-InduciblemBio · 2018Article
- Improved Tet-On and Tet-Off systems for tetracycline-regulated expression of genes in Candida.Current genetics · 2018Article
- Article
- Identification of small molecules that disrupt vacuolar function in the pathogen Candida albicans.PloS one · 2017Article
30 more citing papers are in PubMed but not listed here.
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Abstract
The ura-blaster technique for the disruption of Candida albicans genes has been employed in a number of studies to identify possible genes encoding virulence factors of this fungal pathogen. In this study, the URA3-encoded orotidine 5'-monophosphate (OMP) decarboxylase enzyme activities of C. albicans strains with ura-blaster-mediated genetic disruptions were measured. All strains harboring genetic lesions via the ura-blaster construct showed reduced OMP decarboxylase activities compared to that of the wild type when assayed. The activity levels in different gene disruptions varied, suggesting a positional effect on the level of gene expression. Because the URA3 gene of C. albicans has previously been identified as a virulence factor for this microorganism, our results suggest that decreased virulence observed in strains constructed with the ura-blaster cassette cannot accurately be attributed, in all cases, to the targeted genetic disruption. Although revised methods for validating a URA3-disrupted gene as a target for antifungal drug development could be devised, it is clearly desirable to replace URA3 with a different selectable marker that does not influence virulence.
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