ArticleAnalytical biochemistry1998
The maltose-binding protein as a scaffold for monovalent display of peptides derived from phage libraries.
Article in Analytical biochemistry, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 46 citations in OpenAlex.
- Structural basis of porcine reproductive and respiratory syndrome virus 2 neutralization by a GP4-targeting monoclonal antibody.The Journal of general virology · 2026Article
- Inhibiting complex IL-17A and IL-17RA interactions with a linear peptide.Scientific reports · 2016Article
- Direct expression and validation of phage-selected peptide variants in mammalian cells.The Journal of biological chemistry · 2013Article
- Exploring peptide mimics for the production of antibodies against discontinuous protein epitopes.Molecular immunology · 2010Article
- A peptide inhibitor of HIV-1 neutralizing antibody 2G12 is not a structural mimic of the natural carbohydrate epitope on gp120.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Article
- High-throughput method for ranking the affinity of peptide ligands selected from phage display libraries.Bioconjugate chemistry · 2008Article
- Structure of a high-affinity "mimotope" peptide bound to HIV-1-neutralizing antibody b12 explains its inability to elicit gp120 cross-reactive antibodies.Journal of molecular biology · 2007Article
- In vivo analysis of an essential archaeal signal recognition particle in its native host.Journal of bacteriology · 2002Article
- Random-peptide libraries and antigen-fragment libraries for epitope mapping and the development of vaccines and diagnostics.Current opinion in chemical biology · 2001Review
- Epitope mapping porcine reproductive and respiratory syndrome virus by phage display: the nsp2 fragment of the replicase polyprotein contains a cluster of B-cell epitopes.Journal of virology · 2001Article
- The efficiency of Escherichia coli selenocysteine insertion is influenced by the immediate downstream nucleotide.Nucleic acids research · 2000Article
- Development of a small peptide tag for covalent labeling of proteins.Bioconjugate chemistryArticle
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Random peptide libraries are displayed on filamentous bacteriophage as fusions to either the minor coat protein, pIII, or the major coat protein, pVIII. We have devised a means of isolating the peptide displayed on a phage clone by transferring it to the N-terminus of the maltose-binding protein (MBP) of Escherichia coli encoded by malE. Transfer of a peptide sequence to monomeric MBP eliminates phage-encoded amino acids downstream of the insert peptide as well as avidity effects caused by multivalent display on phage. Peptide:MBP fusions are also easily affinity purified on amylose columns. The pMal-p2 vector was engineered to accept phage DNA encoding pIII- and pVIII-displayed peptides fused to their respective leader sequences. Both types of leader sequence were shown to target the peptide:MBP fusions to the periplasm of E. coli. A streamlined procedure for transferring peptides to MBP was applied to clones that had been isolated from a panel of pVIII-displayed peptide libraries by screening with an HIV-1-specific monoclonal antibody (Ab). By enzyme-linked immunosorbent assay, the Ab bound each of the peptide:MBP fusions and required the presence of a disulfide bridge within each peptide. Some of the peptide:MBP fusions were also analyzed using surface plasmon resonance. Thus, our study shows the value of malE fusion vectors in characterizing phage-displayed peptides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.