ArticleThe Journal of physiology1998
Transmitter release modulation in nerve terminals of rat neocortical pyramidal cells by intracellular calcium buffers.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 90 citations in OpenAlex.
- Modeling and simulation of neocortical micro- and mesocircuitry (Part II, Physiology and experimentation).eLife · 2026Article
- Physiology of intracellular calcium buffering.Physiological reviews · 2023Review
- Roles and Sources of Calcium in Synaptic Exocytosis.Advances in neurobiology · 2023Article
- Upregulated CaCells · 2022Article
- Developmental and activity-dependent modulation of coupling distance between release site and CaFrontiers in cellular neuroscience · 2022Review
- Perinatal interference with the serotonergic system affects VTA function in the adult via glutamate co-transmission.Molecular psychiatry · 2021Article
- Presynaptic voltage-gated calcium channels in the auditory brainstem.Molecular and cellular neurosciences · 2021Review
- Presynaptic endoplasmic reticulum regulates short-term plasticity in hippocampal synapses.Communications biology · 2021Article
- Interstitial ions: A key regulator of state-dependent neural activity?Progress in neurobiology · 2020Review
- Mechanisms and Functional Consequences of Presynaptic Homeostatic Plasticity at Auditory Nerve Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Suppression of Presynaptic Glutamate Release by Postsynaptic Metabotropic NMDA Receptor Signalling to Pannexin-1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Synaptotagmin CaFrontiers in molecular neuroscience · 2018Review
- Structural Properties of Synaptic Transmission and Temporal Dynamics at Excitatory Layer 5B Synapses in the Adult Rat Somatosensory Cortex.Frontiers in synaptic neuroscience · 2018Article
- Intracellular,The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Spontaneous Vesicle Release Is Not Tightly Coupled to Voltage-Gated Calcium Channel-Mediated Ca2+ Influx and Is Triggered by a Ca2+ Sensor Other Than Synaptotagmin-2 at the Juvenile Mice Calyx of Held Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Developmental tightening of cerebellar cortical synaptic influx-release coupling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Anatomy and physiology of the thick-tufted layer 5 pyramidal neuron.Frontiers in cellular neuroscience · 2015Review
- Layer Specific Development of Neocortical Pyramidal to Fast Spiking Cell Synapses.Frontiers in cellular neuroscience · 2015Article
- Article
- Morphology and physiology of excitatory neurons in layer 6b of the somatosensory rat barrel cortex.Cerebral cortex (New York, N.Y. : 1991) · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. Dual whole-cell voltage recordings were made from synaptically connected layer 5 (L5) pyramidal neurones in slices of the young (P14-P16) rat neocortex. The Ca2+ buffers BAPTA or EGTA were loaded into the presynaptic neurone via the pipette recording from the presynaptic neurone to examine their effect on the mean and the coefficient of variation (c.v.) of single fibre EPSP amplitudes, referred to as unitary EPSPs. 2. The fast Ca2+ buffer BAPTA reduced unitary EPSP amplitudes in a concentration dependent way. With 0.1 mM BAPTA in the pipette, the mean EPSP amplitude was reduced by 14 +/- 2.8% (mean +/- s.e.m., n = 7) compared with control pipette solution, whereas with 1.5 mM BAPTA, the mean EPSP amplitude was reduced by 72 +/- 1.5% (n = 5). The concentration of BAPTA that reduced mean EPSP amplitudes to one-half of control was close to 0.7 mM. 3. Saturation of BAPTA during evoked release was tested by comparing the effect of loading the presynaptic neurone with 0.1 mM BAPTA at 2 and 1 mM [Ca2+]o. Reducing [Ca2+]o from 2 to 1 mM, thereby reducing Ca2+ influx into the terminals, decreased the mean EPSP amplitude by 60 +/- 2.2% with control pipette solution and by 62 +/- 1.9% after loading with 0.1 mM BAPTA (n = 7). 4. The slow Ca2+ buffer EGTA at 1 mM reduced mean EPSP amplitudes by 15 +/- 2.5% (n = 5). With 10 mM EGTA mean EPSP amplitudes were reduced by 56 +/- 2.3 % (n = 4). 5. With both Ca2+ buffers, the reduction in mean EPSP amplitudes was associated with an increase in the c.v. of peak EPSP amplitudes, consistent with a reduction of the transmitter release probability as the major mechanism underlying the reduction of the EPSP amplitude. 6. The results suggest that in nerve terminals of thick tufted L5 pyramidal cells the endogenous mobile Ca2+ buffer is equivalent to less than 0.1 mM BAPTA and that at many release sites of pyramidal cell terminals the Ca2+ channel domains overlap, a situation comparable with that at large calyx-type terminals in the brainstem.
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