ArticleThe Journal of physiology1998
An intercellular regenerative calcium wave in porcine coronary artery endothelial cells in primary culture.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 58 citations in OpenAlex.
- Skin capillary endothelial cells form a network of spatiotemporally conserved CaProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Skin capillary endothelial cells form a network of spatiotemporally conserved CabioRxiv : the preprint server for biology · 2026Article
- The Emerging Role of Endothelial Ion Channels in the Control of Human Microcirculation.International journal of molecular sciences · 2026Review
- Cracking the Endothelial Calcium (CaInternational journal of molecular sciences · 2023Review
- Intercellular Calcium Waves and Permeability Change Induced by Vertically Deployed Surface Acoustic Waves in a Human Cerebral Microvascular Endothelial Cell Line (hCMEC/D3) Monolayer.Ultrasound in medicine & biology · 2023Article
- P-Selectin and ICAM-1 synergy in mediating THP-1 monocyte adhesion in hemodynamic flow is length dependent.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- Intercellular ultrafast Ca(2+) wave in vascular smooth muscle cells: numerical and experimental study.Scientific reports · 2016Article
- The vascular conducted response in cerebral blood flow regulation.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2013Review
- Intercellular Ca(2+) waves: mechanisms and function.Physiological reviews · 2012Review
- Temperature effects on morphological integrity and Ca²⁺ signaling in freshly isolated murine feed artery endothelial cell tubes.American journal of physiology. Heart and circulatory physiology · 2011Article
- Phospholipase C-delta extends intercellular signalling range and responses to injury-released growth factors in non-excitable cells.Cell proliferation · 2008Article
- Connexin 40 and ATP-dependent intercellular calcium wave in renal glomerular endothelial cells.American journal of physiology. Regulatory, integrative and comparative physiology · 2008Article
- Smooth muscle mediates circumferential conduction of hyperpolarization and relaxation to focal endothelial cell activation in large coronary arteries.Naunyn-Schmiedeberg's archives of pharmacology · 2007Article
- Depolarization-induced calcium influx in rat mesenteric small arterioles is mediated exclusively via mibefradil-sensitive calcium channels.British journal of pharmacology · 2004Article
- Intercellular Ca2+ wave propagation through gap-junctional Ca2+ diffusion: a theoretical study.Biophysical journal · 2001Article
- An evaluation of potassium ions as endothelium-derived hyperpolarizing factor in porcine coronary arteries.British journal of pharmacology · 2000Article
- The mechanism mediating regenerative intercellular Ca2+ waves in the blowfly salivary gland.The EMBO journal · 1999Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. A regenerative calcium wave is an increase in cytosolic free calcium concentration ([Ca2+]i) which extends beyond the stimulated cells without decrement of amplitude, kinetics of [Ca2+]i increase and speed of propagation. 2. The aim of the present study was to test the hypothesis that such a wave could be evoked by bradykinin stimulation and by scraping cultured endothelial cells from porcine coronary arteries. 3. Calcium imaging was performed using the calcium-sensitive dye fura-2. A wound or a delivery of bradykinin to two to three cells on growing clusters of approximately 300 cells caused an increase in [Ca2+]i which was propagated throughout the cluster in a regenerative manner over distances up to 400 micrometer. This wave spread through gap junctions since it was inhibited by the cell uncoupler palmitoleic acid. 4. The same experiments performed in confluent cultures caused a rise in [Ca2+]i which failed to propagate in a regenerative way. The wave propagation probably failed because the confluent cells were less dye coupled than the growing cells. This was confirmed by immunohistology which detected a dramatic decrease in the number of connexin 40 gap junctions in the confluent cultures. 5. The regenerative propagation of the wave was blocked by inhibitors of calcium-induced calcium release (CICR) and phospholipase C (PLC), and by suppression of extracellular calcium, but not by clamping the membrane potential with high-potassium solution. 6. We conclude that regenerative intercellular calcium waves exist in cultured islets but not in confluent cultures of endothelial cells. An increase in [Ca2+]i is not sufficient to trigger a regenerative propagation. The PLC pathway, CICR and extracellular calcium are all necessary for a fully regenerated propagation.
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