ArticleMolecular and cellular biology1998
Cse1p is involved in export of yeast importin alpha from the nucleus.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 118 citations in OpenAlex.
- The N-terminal extension of ribosomal protein Rps2 shapes its early fate through Chaperone-Importin competition and arginine methylation.The Journal of biological chemistry · 2026Article
- Unconventional tonicity-regulated nuclear trafficking of NFAT5 mediated by KPNB1, XPOT and RUVBL2.Journal of cell science · 2022Article
- Eukaryotic Ribosome assembly and Nucleocytoplasmic Transport.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Dissecting the roles of Cse1 and Nup2 in classical NLS-cargo release in vivo.Traffic (Copenhagen, Denmark) · 2020Article
- Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2.Nature communications · 2018Article
- Identification of the NovelG3 (Bethesda, Md.) · 2018Article
- Nuclear transport adapts to varying heat stress in a multistep mechanism.The Journal of cell biology · 2018Article
- Prefabrication of a ribosomal protein subcomplex essential for eukaryotic ribosome formation.eLife · 2016Article
- Review
- Article
- Neurospora importin α is required for normal heterochromatic formation and DNA methylation.PLoS genetics · 2015Article
- Components and regulation of nuclear transport processes.The FEBS journal · 2015Review
- A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly.eLife · 2014Article
- Genome rearrangements caused by depletion of essential DNA replication proteins in Saccharomyces cerevisiae.Genetics · 2012Article
- The importin β binding domain as a master regulator of nucleocytoplasmic transport.Biochimica et biophysica acta · 2011Review
- Ran-dependent nuclear export mediators: a structural perspective.The EMBO journal · 2011Review
- Yeast karyopherin Kap95 is required for cell cycle progression at Start.BMC cell biology · 2010Article
- Tubulin chaperone E binds microtubules and proteasomes and protects against misfolded protein stress.Cellular and molecular life sciences : CMLS · 2010Article
- Two isoforms of Npap60 (Nup50) differentially regulate nuclear protein import.Molecular biology of the cell · 2010Article
- The intracellular mobility of nuclear import receptors and NLS cargoes.Biophysical journal · 2009Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteins bearing a nuclear localization signal (NLS) are targeted to the nucleus by the heterodimeric transporter importin. Importin alpha binds to the NLS and to importin beta, which carries it through the nuclear pore complex (NPC). Importin disassembles in the nucleus, evidently by binding of RanGTP to importin beta. The importin subunits are exported separately. We investigated the role of Cse1p, the Saccharomyces cerevisiae homologue of human CAS, in nuclear export of Srp1p (yeast importin alpha). Cse1p is located predominantly in the nucleus but also is present in the cytoplasm and at the NPC. We analyzed the in vivo localization of the importin subunits fused to the green fluorescent protein in wild-type and cse1-1 mutant cells. Srp1p but not importin beta accumulated in nuclei of cse1-1 mutants, which are defective in NLS import but not defective in NLS-independent import pathways. Purified Cse1p binds with high affinity to Srp1p only in the presence of RanGTP. The complex is dissociated by the cytoplasmic RanGTP-binding protein Yrb1p. Combined with the in vivo results, this suggests that a complex containing Srp1p, Cse1p, and RanGTP is exported from the nucleus and is subsequently disassembled in the cytoplasm by Yrb1p. The formation of the trimeric Srp1p-Cse1p-RanGTP complex is inhibited by NLS peptides, indicating that only NLS-free Srp1p will be exported to the cytoplasm.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.