ArticleMolecular and cellular biology1998
Cak1 is required for Kin28 phosphorylation and activation in vivo.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 71 citations in OpenAlex.
- Cyclin-dependent kinases: Masters of the eukaryotic universe.Wiley interdisciplinary reviews. RNA · 2023Review
- A mitogen-activated protein kinase PoxMK1 mediates regulation of the production of plant-biomass-degrading enzymes, vegetative growth, and pigment biosynthesis in Penicillium oxalicum.Applied microbiology and biotechnology · 2021Article
- Gene modification by fast-track recombineering for cellular localization and isolation of components of plant protein complexes.The Plant journal : for cell and molecular biology · 2019Article
- The telomeric Cdc13-Stn1-Ten1 complex regulates RNA polymerase II transcription.Nucleic acids research · 2019Article
- Cdk7: a kinase at the core of transcription and in the crosshairs of cancer drug discovery.Transcription · 2019Review
- Budding yeast Wee1 distinguishes spindle pole bodies to guide their pattern of age-dependent segregation.Nature cell biology · 2017Article
- Rpb4/7 facilitates RNA polymerase II CTD dephosphorylation.Nucleic acids research · 2014Article
- The CDK Network: Linking Cycles of Cell Division and Gene Expression.Genes & cancer · 2012Article
- CDKF;1 and CDKD protein kinases regulate phosphorylation of serine residues in the C-terminal domain of Arabidopsis RNA polymerase II.The Plant cell · 2012Article
- Dissecting phosphorylation networks: lessons learned from yeast.Expert review of proteomics · 2011Review
- Sporulation in the budding yeast Saccharomyces cerevisiae.Genetics · 2011Review
- Acid phosphatases of budding yeast as a model of choice for transcription regulation research.Enzyme research · 2011Article
- Sub1 globally regulates RNA polymerase II C-terminal domain phosphorylation.Molecular and cellular biology · 2010Article
- A phosphorylation-independent role for the yeast cyclin-dependent kinase activating kinase Cak1.Gene · 2009Article
- Article
- In silico construction of a protein interaction landscape for nucleotide excision repair.Cell biochemistry and biophysics · 2009Article
- Article
- Structure of the Pho85-Pho80 CDK-cyclin complex of the phosphate-responsive signal transduction pathway.Molecular cell · 2007Article
- The multiple roles of cyclin E1 in controlling cell cycle progression and cellular morphology of Trypanosoma brucei.Journal of molecular biology · 2007Article
- Mechanisms regulating the protein kinases of Saccharomyces cerevisiae.Eukaryotic cell · 2007Review
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Complete activation of most cyclin-dependent protein kinases (CDKs) requires phosphorylation by the CDK-activating kinase (CAK). In the budding yeast, Saccharomyces cerevisiae, the major CAK is a 44-kDa protein kinase known as Cak1. Cak1 is required for the phosphorylation and activation of Cdc28, a major CDK involved in cell cycle control. We addressed the possibility that Cak1 is also required for the activation of other yeast CDKs, such as Kin28, Pho85, and Srb10. We generated three new temperature-sensitive cak1 mutant strains, which arrested at the restrictive temperature with nonuniform budding morphology. All three cak1 mutants displayed significant synthetic interactions with loss-of-function mutations in CDC28 and KIN28. Loss of Cak1 function reduced the phosphorylation and activity of both Cdc28 and Kin28 but did not affect the activity of Pho85 or Srb10. In the presence of the Kin28 regulatory subunits Ccl1 and Tfb3, Kin28 was phosphorylated and activated when coexpressed with Cak1 in insect cells. We conclude that Cak1 is required for the activating phosphorylation of Kin28 as well as that of Cdc28.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.