ArticleMolecular and cellular biology1998
Evidence that immunoglobulin VH-DJ recombination does not require germ line transcription of the recombining variable gene segment.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 77 citations in OpenAlex.
- RNA exosome drives early B cell development via noncoding RNA processing mechanisms.Science immunology · 2022Article
- Overexpression of wild type RRAS2, without oncogenic mutations, drives chronic lymphocytic leukemia.Molecular cancer · 2022Article
- Article
- Recombination may occur in the absence of transcription in the immunoglobulin heavy chain recombination centre.Nucleic acids research · 2020Article
- A comparison of unamplified and massively multiplexed PCR amplification for murine antibody repertoire sequencing.FASEB bioAdvances · 2019Article
- A comparison of unamplified and massively multiplexed PCR amplification for murine antibody repertoire sequencing.FASEB bioAdvances · 2019Article
- LAMTOR2 (p14) Controls B Cell Differentiation by Orchestrating Endosomal BCR Trafficking.Frontiers in immunology · 2019Article
- Cutting Edge: Proper Orientation of CTCF Sites in Cer Is Required for Normal Jκ-Distal and Jκ-Proximal Vκ Gene Usage.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Corrected and Republished from: BCL11A Is a Critical Component of a Transcriptional Network That Activates RAG Expression and V(D)J Recombination.Molecular and cellular biology · 2018Article
- Evolutionary paradox of immunity.North American journal of medical sciences · 2015Article
- The BCL11A transcription factor directly activates RAG gene expression and V(D)J recombination.Molecular and cellular biology · 2013Article
- Interaction between the immunoglobulin heavy chain 3' regulatory region and the IgH transcription unit during B cell differentiation.Molecular immunology · 2011Article
- The ARID family transcription factor bright is required for both hematopoietic stem cell and B lineage development.Molecular and cellular biology · 2011Article
- Control of V(D)J Recombination through Transcriptional Elongation and Changes in Locus Chromatin Structure and Nuclear Organization.Genetics research international · 2011Article
- Two distinct populations of H chain-edited B cells show differential surrogate L chain dependence.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- The epigenetic profile of Ig genes is dynamically regulated during B cell differentiation and is modulated by pre-B cell receptor signaling.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Activation of 12/23-RSS-dependent RAG cleavage by hSWI/SNF complex in the absence of transcription.Molecular cell · 2008Article
- Requirement for enhancer specificity in immunoglobulin heavy chain locus regulation.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Cis-regulatory elements and epigenetic changes control genomic rearrangements of the IgH locus.Advances in immunology · 2008Review
- B cell-specific loss of histone 3 lysine 9 methylation in the V(H) locus depends on Pax5.Nature immunology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The importance of V(D)J recombination for generating diversity in the immune system is well established, but the mechanisms which regulate V(D)J recombination are still poorly understood. Although transcription of unrearranged (germ line) immunoglobulin and T-cell receptor gene segments often precedes V(D)J recombination and has been implicated in its control, the actual role of germ line transcripts in V(D)J recombination is not known. We used a sensitive reverse transcription-PCR assay to study immunoglobulin VH germ line transcripts in proB lines from RAG-deficient mice. All 10 VH families analyzed were germ line transcribed, and germ line transcription was found in all of the cell lines examined, indicating that active chromatin was present in the VH region. However, not all VH families were germ line transcribed in every cell line, and there was a surprising lack of uniformity in the number and family distribution of germ line VH transcripts in individual lines. When V(D)J recombination was activated by restoration of RAG activity, recombinational activity of endogenous VH genes for which germ line transcription was observed could be compared with those of genes for which it was not observed. This analysis revealed multiple examples of endogenous VH gene segments which were rearranged in cells where their germ line transcription was not detectable prior to RAG expression. Thus, our data provide strong support for the idea that V-(D)J recombination does not require germ line transcription of the recombining variable gene segment.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.