Evidence map›Paper›PMID 9774618›Full record

ArticleJournal of clinical microbiology1998

Development of a nested PCR for detection of Cryptococcus neoformans in cerebrospinal fluid.

P Rappelli, R Are, G Casu, P L Fiori, P Cappuccinelli, A Aceti

Abstract read
In one paragraph

Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Cryptococcus gattii infections.Clinical microbiology reviews · 2014
    Review
  10. Review
  11. Article
  12. Review
  13. Article
  14. Review
  15. Article
  16. Review
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

P RappelliDepartment of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy. micropat@ssmain.uniss.it
R Are
G Casu
P L Fiori
P Cappuccinelli
A Aceti

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report the development of a nested-PCR-based assay for the detection of Cryptococcus neoformans in cerebrospinal fluid. The specificity and sensitivity of the test were assessed. The technique was then applied to 40 cerebrospinal fluid samples. We obtained positive reactions for all 21 clinical samples from patients who had been previously diagnosed as having cryptococcal meningitis by conventional techniques and negative reactions for all 19 negative controls. Nested PCR is here compared with other diagnostic methods currently used in patients' follow-up exams during anticryptococcal therapy.

Indexed as

Base SequenceCerebrospinal FluidCryptococcosisCryptococcus neoformansDNA PrimersEvaluation Studies as TopicHumansMycologyPolymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificityDNA Primers

Identifiers

PMID9774618
PMCPMC105354

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.