ArticleJournal of clinical microbiology1998
Direct detection of respiratory syncytial virus, parainfluenza virus, and adenovirus in clinical respiratory specimens by a multiplex reverse transcription-PCR assay.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
68 citing papers in PubMed, 1 synthesis or guideline pooled it, 139 citations in OpenAlex.
- Parainfluenza virus infections in hematopoietic cell transplant recipients and hematologic malignancy patients: A systematic review.Cancer letters · 2016Pooled it
- Role of picornaviruses in flu-like illnesses of adults enrolled in an oseltamivir treatment study who had no evidence of influenza virus infection.Journal of clinical microbiology · 2002Trial
- Influenza and other respiratory viruses in children: prevalence and clinical features.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2022Article
- Viral Respiratory Infections in Hematological Patients.Infectious diseases and therapy · 2020Review
- Diversity of respiratory viruses detected among hospitalized children with acute lower respiratory tract infections at Hospital Serdang, Malaysia.Journal of virological methods · 2019Article
- The Impact of RSV-Associated Respiratory Disease on Children in Asia.Journal of pediatric infectious diseases · 2019Review
- Simulation of four respiratory viruses and inference of epidemiological parameters.Infectious Disease Modelling · 2018Article
- Viral Pneumonia in Patients with Hematopoietic Cell Transplantation and Hematologic Malignancies.Clinics in chest medicine · 2017Review
- Parainfluenza Virus Infection.Seminars in respiratory and critical care medicine · 2016Review
- Significant Transplantation-Related Mortality from Respiratory Virus Infections within the First One Hundred Days in Children after Hematopoietic Stem Cell Transplantation.Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation · 2015Article
- Current and Prospective Methods for Plant Disease Detection.Biosensors · 2015Review
- Viral coinfection in acute respiratory infection in Mexican children treated by the emergency service: A cross-sectional study.Italian journal of pediatrics · 2015Article
- Management of respiratory viral infections in hematopoietic cell transplant recipients and patients with hematologic malignancies.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014Review
- Another case of respiratory syncytial virus-related limbic encephalitis.Neuroradiology · 2014Article
- Advances in diagnosis of respiratory diseases of small ruminants.Veterinary medicine international · 2014Review
- Etiological analysis and predictive diagnostic model building of community-acquired pneumonia in adult outpatients in Beijing, China.BMC infectious diseases · 2013Article
- Respiratory viral infections after hematopoietic stem cell transplantation in children.Journal of Korean medical science · 2013Article
- Detection of human bocaviruses 1 to 4 from nasopharyngeal swab samples collected from patients with respiratory tract infections.Journal of clinical microbiology · 2012Article
- Viral agents causing lower respiratory tract infections in hospitalized children: evaluation of the Speed-Oligo® RSV assay for the detection of respiratory syncytial virus.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Management of respiratory viral infections in hematopoietic cell transplant recipients.American journal of blood research · 2012Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diagnosis of respiratory virus infections currently involves detection by isolation or antigen detection, which usually identifies only a single suspected agent. To permit identification of more than one respiratory virus in clinical specimens, a rapid detection method involving a single-step, multiplex reverse transcription-PCR (RT-PCR) assay was developed. The assay included five primer sets that amplified the RNA of respiratory syncytial virus subtypes A and B, parainfluenza virus types 1, 2, and 3, and adenovirus types 1 to 7. Initially the assay was tested on tissue culture-grown virus and was found to be specific for all 12 prototype viruses tested, with no interassay cross amplification or amplification of other respiratory viruses. Assay sensitivity allowed a detection range of 0.2 50% tissue culture infectious dose (TCID50) for adenovirus to 250 TCID50 for parainfluenza virus type 1. The multiplex RT-PCR assay was also able to directly detect viruses in respiratory specimens, with virus being detected in 41 of 112 samples as compared to 34 of 112 samples detected by direct immunofluorescence or antigen detection following specimen culture. This suggests that the multiplex RT-PCR assay can be used as a rapid and sensitive diagnostic method for major respiratory viruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.