ArticleJournal of clinical microbiology1998
Fluorescent amplified-fragment length polymorphism analysis of an outbreak of group A streptococcal invasive disease.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 71 citations in OpenAlex.
- Generation of metabolically diverse strains of Streptococcus pyogenes during survival in stationary phase.Journal of bacteriology · 2009Article
- Identification of Clostridium species and DNA fingerprinting of Clostridium perfringens by amplified fragment length polymorphism analysis.Journal of clinical microbiology · 2006Article
- M protein typing of Thai group A streptococcal isolates by PCR-Restriction fragment length polymorphism analysis.BMC microbiology · 2005Article
- A normalization strategy applied to HiCEP (an AFLP-based expression profiling) analysis: toward the strict alignment of valid fragments across electrophoretic patterns.BMC bioinformatics · 2005Article
- Efficient DNA fingerprinting of Clostridium botulinum types A, B, E, and F by amplified fragment length polymorphism analysis.Applied and environmental microbiology · 2005Article
- Fluorescent amplified fragment length polymorphism genotyping of Campylobacter jejuni and Campylobacter coli strains and its relationship with host specificity, serotyping, and phage typing.Journal of clinical microbiology · 2004Article
- Fine-structure molecular typing of Irish Helicobacter pylori isolates and their genetic relatedness to strains from four different continents.Journal of clinical microbiology · 2003Article
- Genotyping of Francisella tularensis strains by pulsed-field gel electrophoresis, amplified fragment length polymorphism fingerprinting, and 16S rRNA gene sequencing.Journal of clinical microbiology · 2002Article
- Development of a procedure for discriminating among Escherichia coli isolates from animal and human sources.Applied and environmental microbiology · 2002Article
- Genome sequence-based fluorescent amplified fragment length polymorphism of Campylobacter jejuni, its relationship to serotyping, and its implications for epidemiological analysis.Journal of clinical microbiology · 2001Article
- Genotyping Neisseria gonorrhoeae using fluorescent amplified fragment length polymorphism analysis.Journal of clinical microbiology · 2001Article
- Molecular typing and epidemiological study of Salmonella enterica serotype Typhimurium isolates from cattle by fluorescent amplified-fragment length polymorphism fingerprinting and pulsed-field gel electrophoresis.Journal of clinical microbiology · 2001Article
- Fluorescent amplified-fragment length polymorphism subtyping of the Salmonella enterica serovar enteritidis phage type 4 clone complex.Journal of clinical microbiology · 2001Article
- Genotyping of verocytotoxin-producing Escherichia coli O157: comparison of isolates of a prevalent phage type by fluorescent amplified-fragment length polymorphism and pulsed-field gel electrophoresis analyses.Journal of clinical microbiology · 2000Article
- Fluorescent amplified-fragment length polymorphism genotyping of Neisseria meningitidis identifies clones associated with invasive disease.Journal of clinical microbiology · 2000Article
- Evaluation of fluorescence-based amplified fragment length polymorphism analysis for molecular typing in hospital epidemiology: comparison with pulsed-field gel electrophoresis for typing strains of vancomycin-resistant Enterococcus faecium.Journal of clinical microbiology · 2000Article
- Fluorescent amplified-fragment length polymorphism genotyping of Salmonella enterica subsp. enterica serovars and comparison with pulsed-field gel electrophoresis typing.Journal of clinical microbiology · 2000Article
- Genotyping of epidemic methicillin-resistant Staphylococcus aureus phage type 15 isolates by fluorescent amplified-fragment length polymorphism analysis.Journal of clinical microbiology · 1999Article
- Amplified-fragment length polymorphism fingerprinting of Mycoplasma species.Journal of clinical microbiology · 1999Article
- Amplified-fragment length polymorphism analysis: the state of an art.Journal of clinical microbiology · 1999Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent amplified-fragment length polymorphism (FAFLP) analysis was carried out for an outbreak of group A streptococcal (GAS) invasive disease. Streptococcal genomic DNAs were digested with endonucleases EcoRI and MseI, site-specific adaptors were ligated, and PCR amplification was carried out with an EcoRI adaptor-specific primer labelled with fluorescent dye. Amplified fragments of up to 600 bp in size were separated on a polyacrylamide sequencing gel which contained internal size markers in each lane. These data were automatically scanned and analyzed, fragments were precisely sized (+/-1 bp), and electropherograms were generated for each genome with GeneScan 2.1 software. All isolates were compared in this way. Among 27 GAS isolates examined, we found 18 FAFLP profiles, compared with 12 macrorestriction profiles by pulsed-field gel electrophoresis. FAFLP readily distinguished genotypes for two clones of GAS serotype M77 which were responsible for outbreaks of invasive disease in a care-of-the-elderly system. It provided an automated analysis of the whole genome of bacterial isolates. It was reproducible, more discriminatory, and capable of higher throughput than other molecular typing methods. Given agreed conditions, FAFLP would be reproducible between laboratories for rapid characterization of outbreak strains.
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