ArticleThe EMBO journal1998
Capture of genomic and T-DNA sequences during double-strand break repair in somatic plant cells.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 145 papers.
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Who cites it
145 citing papers in PubMed, 358 citations in OpenAlex.
- Arabidopsis DNA repair mutants can integrate Agrobacterium T-DNA into the plant genome.The New phytologist · 2026Article
- Loss-of-function of MIR172b and prime editing of SNB reveal a regulatory module underlying cleistogamy in rice.Plant physiology · 2026Article
- Single-Cell CRISPR: An Efficient Strategy for Decoding Plant Cis-Regulatory Complexity.Plant biotechnology journal · 2026Review
- Variable alterations in frequencies of CRISPR/Cas9-induced nucleotide deletion and insertion in transgenic maize callus tissues exposed to chromatin modifiers.Frontiers in genome editing · 2026Article
- The evolving landscape of precise DNA insertion in plants.Nature communications · 2025Review
- Versatile Applications of CRISPR-Based Programmable T-DNA Integration in Plants.Plant biotechnology journal · 2025Article
- Article
- Characterization of radiations-induced genomic structural variations in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2025Article
- Genetic dissection of mutagenic repair and T-DNA capture at CRISPR-induced DNA breaks inPNAS nexus · 2024Article
- Genomic consequences associated with Agrobacterium-mediated transformation of plants.The Plant journal : for cell and molecular biology · 2024Review
- Reproductive defects in the abscission mutant ida-2 are caused by T-DNA-induced genomic rearrangements.Plant physiology · 2023Article
- Identification of T-DNA structure and insertion site in transgenic crops using targeted capture sequencing.Frontiers in plant science · 2023Article
- High-throughput sgRNA testing reveals rules for Cas9 specificity and DNA repair in tomato cells.Frontiers in genome editing · 2023Article
- CRISPR/Cas9-Mediated Targeted DNA Integration: Rearrangements at the Junction of Plant and Plasmid DNA.International journal of molecular sciences · 2022Article
- Risk assessment of a new bioinformatics evaluation of the insertion sites of genetically modified soybean event 40-3-2.EFSA journal. European Food Safety Authority · 2022Article
- Efficient gene targeting in soybean using Ochrobactrum haywardense-mediated delivery of a marker-free donor template.Plant physiology · 2022Article
- CRISPR-Cas9-mediated chromosome engineering in Arabidopsis thaliana.Nature protocols · 2022Review
- Review
- Nonhomologous end joining as key to CRISPR/Cas-mediated plant chromosome engineering.Plant physiology · 2022Article
- Review
85 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To analyze genomic changes resulting from double-strand break (DSB) repair, transgenic tobacco plants were obtained that carried in their genome a restriction site of the rare cutting endonuclease I-SceI within a negative selectable marker gene. After induction of DSB repair via Agrobacterium-mediated transient expression of I-SceI, plant cells were selected that carried a loss-of-function phenotype of the marker. Surprisingly, in addition to deletions, in a number of cases repair was associated with the insertion of unique and repetitive genomic sequences into the break. Thus, DSB repair offers a mechanism for spreading different kinds of sequences into new chromosomal positions. This may have evolutionary consequences particularly for plants, as genomic alterations occurring in meristem cells can be transferred to the next generation. Moreover, transfer DNA (T-DNA), carrying the open reading frame of I-SceI, was found in several cases to be integrated into the transgenic I-SceI site. This indicates that DSB repair also represents a pathway for the integration of T-DNA into the plant genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.