ArticleThe EMBO journal1998
Gelsolin and functionally similar actin-binding proteins are regulated by lysophosphatidic acid.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The trial behind it
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Who cites it
29 citing papers in PubMed, 94 citations in OpenAlex.
- Coupled Electrostatic and Hydrophobic Destabilisation of the Gelsolin-Actin Complex Enables Facile Detection of Ovarian Cancer Biomarker Lysophosphatidic Acid.Biomolecules · 2023Article
- Entering, Linked with the Sphinx: Lysophosphatidic Acids Everywhere, All at Once, in the Oral System and Cancer.International journal of molecular sciences · 2023Review
- Article
- Lysophosphatidic acid type 2 receptor agonists in targeted drug development offer broad therapeutic potential.Journal of lipid research · 2019Review
- Platelets, autotaxin and lysophosphatidic acid signalling: win-win factors for cancer metastasis.British journal of pharmacology · 2018Review
- Podocalyxin-like protein, linked to poor prognosis of pancreatic cancers, promotes cell invasion by binding to gelsolin.Cancer science · 2016Article
- Heterodimeric capping protein from Arabidopsis is a membrane-associated, actin-binding protein.Plant physiology · 2014Article
- Nm23-h1 binds to gelsolin and inactivates its actin-severing capacity to promote tumor cell motility and metastasis.Cancer research · 2013Article
- Change in blood gelsolin concentration in response to physical exercise.Biology of sport · 2013Article
- Gelsolin in cerebrospinal fluid as a potential biomarker of epilepsy.Neurochemical research · 2011Article
- The protective effects of plasma gelsolin on stroke outcome in rats.Experimental & translational stroke medicine · 2011Article
- Plasma gelsolin facilitates interaction between β2 glycoprotein I and α5β1 integrin.Journal of cellular and molecular medicine · 2011Article
- Plasma gelsolin modulates cellular response to sphingosine 1-phosphate.American journal of physiology. Cell physiology · 2010Article
- Aiming drug discovery at lysophosphatidic acid targets.British journal of pharmacology · 2010Review
- A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction.Cellular and molecular life sciences : CMLS · 2010Article
- Differential effects of lysophosphatidic acid and phosphatidylinositol 4,5-bisphosphate on actin dynamics by direct association with the actin-binding protein villin.The Journal of biological chemistry · 2009Article
- Lysophosphatidic acid and autotaxin: emerging roles in innate and adaptive immunity.Immunologic research · 2009Review
- Roles of lysophosphatidic acid in cardiovascular physiology and disease.Biochimica et biophysica acta · 2008Review
- Decreased levels of the gelsolin plasma isoform in patients with rheumatoid arthritis.Arthritis research & therapy · 2008Article
- Heterodimeric capping protein from Arabidopsis is regulated by phosphatidic acid.Molecular biology of the cell · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An extensive survey was carried out for compounds capable of regulating actin-binding proteins in a manner similar to phosphatidylinositol 4,5 bisphosphate (PI 4,5-P2). For this purpose we developed a sensitive assay involving release of radioactively phosphorylated actin from the fragminP-actin complex. We found that the structurally simplest lysophospholipid, lysophosphatidic acid (LPA), dissociated the complex between fragminP and actin, whereas other lysophospholipids or sphingosine-1-phosphate were inactive. Furthermore, LPA inhibited the F-actin severing activity of human gelsolin, purified from plasma or as recombinant protein, mouse adseverin and Physarum fragminP. Dissociation of actin-containing complexes by LPA analyzed by gelfiltration indicated that LPA is active as a monomer, in contrast to PI 4,5-P2. We further show that binding of LPA to these actin-regulatory proteins promotes their phosphorylation by pp60(c-src). A PI 4,5-P2-binding peptide counteracted the effects mediated by LPA, suggesting that LPA binds to the same target region in these actin-binding proteins. When both LPA and PI 4,5-P2 were used in combination we found that LPA reduced the threshold concentration at which PI 4,5-P2 was active. Significantly, LPA promoted the release of gelsolin from barbed actin filaments in octylglucoside-permeabilized human platelets. These results suggest that lysophosphatidic acid could act as an intracellular modulator of actin-binding proteins. Our findings can also explain agonist-induced changes in the actin cytoskeleton that are not mediated by polyphosphoinositides.
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