Evidence map›Paper›PMID 9774179›Full record

ArticleMolecular and cellular biochemistry1998

Opioid receptor contributes to ischemic preconditioning through protein kinase C activation in rabbits.

T Miki, M V Cohen, J M Downey

Abstract read
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In one paragraph

Article in Molecular and cellular biochemistry, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 158 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

T MikiDepartment of Physiology, University of South Alabama, College of Medicine, Mobile 36688, USA.
M V Cohen
J M Downey
University of South Alabama · US

Funding

PHYSICAL FACTORS AND CORONARY FLOWR01HL020648 · NHLBI · UNIVERSITY OF SOUTH ALABAMA · PI DOWNEY, JAMES M · 1985 to 2011
$2.7M
CATECHOLS AND ADENOSINE IN MYOCARDIAL PRECONDITIONINGR01HL050688 · NHLBI · UNIVERSITY OF SOUTH ALABAMA · PI COHEN, MICHAEL VICTOR · 1993 to 2006
$1.2M
NHLBI NIH HHS HL-20648NHLBI NIH HHS HL-50688
6 · The paper itself

Abstract

Recent studies have reported that protection from ischemic preconditioning (PC) is blocked by the opioid receptor antagonist naloxone (NAL). We tested whether an opioid agonist could mimic PC in the rabbit heart, whether that protection involved protein kinase C (PKC) activation, and whether opioid receptors act in concert with other PKC-coupled receptors. Rabbit hearts were subjected to 30 min coronary occlusions and were reperfused for either 3 (in situ) or 2 (in vitro) h. Infarct size was determined by staining with triphenyltetrazolium chloride. In untreated in situ hearts 38.5+/-1.6% of the risk zone infarcted. PC with 5 min ischemia/10 min reperfusion significantly limited infarction to 12.7+/-2.9% (p < 0.01). NAL infusion did not modify infarction (39.6+/-1.6%) in non-PC hearts, but blocked the effect of one cycle of PC (34.4+/-3.6% infarction). NAL, however, could not block cardioprotection when PC was amplified with 3 cycles of ischemia/reperfusion (9.9+/-1.4% infarction, p < 0.01 vs. control). Morphine could also mimic ischemic preconditioning, but only at a dose much higher than would be used clinically (3 mg/kg). In isolated hearts pretreatment with morphine (0.3 microM) significantly limited infarction to 9.3+/-1.2% (p < 0.01 vs. 32.0+/-3.1% in controls). This cardioprotective effect of morphine could be blocked by either the PKC inhibitor chelerythrine (30.4+/-2.6% infarction) or NAL (34.0+/-2.6% infarction). Neither chelerythrine nor NAL by itself modified infarction in non-PC hearts. NAL could not block protection from one cycle of PC in isolated hearts indicating that an intact innervation may be required for endogenous opioid production. Thus, opioid receptors, like other PKC-coupled receptors, participate in the triggering of PC in the rabbit heart.

Indexed as

Ischemic Preconditioning, MyocardialAlkaloidsAnimalsBenzophenanthridinesEnzyme ActivationEnzyme InhibitorsFemaleIn Vitro TechniquesMaleMorphineMyocardial InfarctionMyocardial IschemiaMyocardial Reperfusion InjuryNaloxoneNarcotic AntagonistsPhenanthridinesAlkaloidsBenzophenanthridineschelerythrineEnzyme InhibitorsMorphineNaloxoneNarcotic AntagonistsPhenanthridinesProtein Kinase CReceptors, Opioid

Identifiers

PMID9774179
OpenAlexW170798846

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.