ArticleGut1998
Expression of inducible nitric oxide synthase activity in human colon epithelial cells: modulation by T lymphocyte derived cytokines.
Article in Gut, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 148 citations in OpenAlex.
- Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus nonresponders.World journal of gastroenterology · 2006Trial
- The hidden piece in inflammatory bowel diseases: The significance of intestinal mucus in mediating the bacteria-cells crosstalk.Materials today. Bio · 2025Review
- Elevated nitric oxide during colitis restrains GM-CSF production in ILC3 cells via suppressing an AhR-Cyp4f13-NF-κB axis.Nature communications · 2025Article
- The Complexity of Interferon Signaling in Host Defense against Protozoan Parasite Infection.Pathogens (Basel, Switzerland) · 2023Review
- Involvement of nitrergic neurons in colonic motility in a rat model of ulcerative colitis.World journal of gastroenterology · 2022Article
- Bile acid-sensitive tuft cells regulate biliary neutrophil influx.Science immunology · 2022Article
- Stressor-Induced Increases in Circulating, but Not Colonic, Cytokines Are Related to Anxiety-like Behavior and Hippocampal Inflammation in a Murine Colitis Model.International journal of molecular sciences · 2022Article
- Cancer-protective effect of a synbiotic combination betweenGut microbes · 2020Article
- Transcriptional and Metabolomic Analysis of L-Arginine/Nitric Oxide Pathway in Inflammatory Bowel Disease and Its Association with Local Inflammatory and Angiogenic Response: Preliminary Findings.International journal of molecular sciences · 2020Article
- PD123319, angiotensin II type II receptor antagonist, inhibits oxidative stress and inflammation in 2, 4-dinitrobenzene sulfonic acid-induced colitis in rat and ameliorates colonic contractility.Inflammopharmacology · 2020Article
- Article
- Inducible nitric oxide synthase-derived nitric oxide reduces vagal satiety signalling in obese mice.The Journal of physiology · 2019Article
- Antibiotics suppress colon tumorigenesis through inhibition of aberrant DNA methylation in an azoxymethane and dextran sulfate sodium colitis model.Cancer science · 2019Article
- Tumor necrosis factorPhysiological reports · 2017Article
- Molecular topology: a strategy to identify novel compounds against ulcerative colitis.Molecular diversity · 2017Article
- Arginine Metabolism in Myeloid Cells Shapes Innate and Adaptive Immunity.Frontiers in immunology · 2017Review
- Roles of nitric oxide and intestinal microbiota in the pathogenesis of necrotizing enterocolitis.Journal of pediatric surgery · 2016Review
- Intestinal myofibroblasts produce nitric oxide in response to combinatorial cytokine stimulation.Journal of cellular physiology · 2013Article
- Thrombospondin-1 and VEGF in inflammatory bowel disease.The Libyan journal of medicine · 2012Article
- Increased expression of chemokine receptor CCR3 and its ligands in ulcerative colitis: the role of colonic epithelial cells in in vitro studies.Clinical and experimental immunology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundNitric oxide (NO) synthesis and inducible nitric oxide synthase (iNOS) expression are increased in colonic biopsy specimens from patients with ulcerative colitis, but the cellular source of NO production is not known.
aimsTo examine the distribution of iNOS in human colonic mucosa and to explore the ability of T lymphocyte derived cytokines to regulate iNOS expression and activity in human colonic epithelial cells.
methodsiNOS expression was examined using immunohistochemistry in colonic biopsy samples from 12 patients with ulcerative colitis and three with infectious colitis and compared with 10 normal controls. In vitro iNOS expression and activity were determined in HT-29 cell cultures; nitrite levels were measured using a fluorescent substrate, iNOS mRNA expression by northern blot analysis, and iNOS protein expression by western blot analysis.
resultsNo iNOS expression was detected (10 of 10) in non-inflamed mucosa derived from normal controls. In 11 of 12 cases of newly diagnosed ulcerative colitis, iNOS protein was expressed in the epithelial cells, while no other positive cells were found in the lamina propria. Similar iNOS labelling was found in colonic biopsy samples from patients with infectious colitis in the acute phase, but when re-examined in samples from patients in total remission, no iNOS staining was observed. Both interleukin (IL)-13 and IL-4, but not IL-10, are potent inhibitors of iNOS expression and activity induced by an optimal combination of cytokines, namely IL-1 alpha, tumour necrosis factor alpha and interferon gamma.
conclusionsThe data suggest that the epithelium is the major source of iNOS activity in ulcerative colitis and that IL-13 and IL-4 may act as intrinsic regulators of NO generation in intestinal inflammation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.